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A Chemical Screen Identifies Compounds Capable of Selecting for Haploidy in Mammalian Cells

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dc.contributor.authorOlbrich, Teresa
dc.contributor.authorVega-Sendino, Maria
dc.contributor.authorMurga, Matilde
dc.contributor.authorde Carcer Diez, Guillermo
dc.contributor.authorMalumbres Martinez, Marcos
dc.contributor.authorOrtega Jimenez, Sagrario
dc.contributor.authorRuiz, Sergio
dc.contributor.authorFernandez-Capetillo, Oscar
dc.contributor.funderBoehringer Ingelheim Fonds
dc.contributor.funderMinisterio de Economía y Competitividad (España)
dc.contributor.funderBotín Foundation
dc.contributor.funderUnión Europea. Comisión Europea. European Research Council (ERC)
dc.date.accessioned2019-08-12T10:21:08Z
dc.date.available2019-08-12T10:21:08Z
dc.date.issued2019-07-16
dc.description.abstractThe recent availability of somatic haploid cell lines has provided a unique tool for genetic studies in mammals. However, the percentage of haploid cells rapidly decreases in these cell lines, which we recently showed is due to their overgrowth by diploid cells present in the cultures. Based on this property, we have now performed a phenotypic chemical screen in human haploid HAP1 cells aiming to identify compounds that facilitate the maintenance of haploid cells. Our top hit was 10-Deacetyl-baccatin-III (DAB), a chemical precursor in the synthesis of Taxol, which selects for haploid cells in HAP1 and mouse haploid embryonic stem cultures. Interestingly, DAB also enriches for diploid cells in mixed cultures of diploid and tetraploid cells, including in the colon cancer cell line DLD-1, revealing a general strategy for selecting cells with lower ploidy in mixed populations of mammalian cells.es_ES
dc.description.peerreviewedSíes_ES
dc.description.sponsorshipWe would like to thank the members of the O.F.-C. laboratory and MonicaAlvarez-Fernandez for insightful comments and the Transgenic Mice, FlowCytometry, and Confocal Microscopy Units from the CNIO for their technicalhelp. T.O. was funded by a PhD fellowship from Boehringer IngelheimFonds. Research was funded by Fundacion Botı n, Banco Santander throughits Santander Universities Global Division, and by grants from MINECO(SAF2014-57791-REDC and SAF2014-59498-R to O.F.-C., SAF-2013-44866-R to S.O., and SAF2013-49147-P and SAF2016-80874-P to S.R.; pro-jects that were co-financed with ERDF-EU funds) and the EuropeanResearch Council (ERC-617840). Research at the G.d.C. laboratory is fundedby the AECC Scientific Foundation (LABAE16017DECA).es_ES
dc.format.number3es_ES
dc.format.page597-604.e4es_ES
dc.format.volume28es_ES
dc.identifier.citationCell Rep. 2019;28(3):597-604.es_ES
dc.identifier.doi10.1016/j.celrep.2019.06.060es_ES
dc.identifier.e-issn2211-1247es_ES
dc.identifier.issn22111247es_ES
dc.identifier.journalCell reportses_ES
dc.identifier.pubmedID31315040es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/8208
dc.language.isoenges_ES
dc.publisherCell Press
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2014-57791-REDCes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/ERC-617840es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2014-59498-Res_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF-2013-44866-Res_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2013-49147-Pes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2016-80874-Pes_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.celrep.2019.06.060.es_ES
dc.repisalud.institucionCNIOes_ES
dc.repisalud.orgCNIOCNIO::Grupos de investigación::Grupo de Inestabilidad Genómicaes_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject10-Deacetylbaccatin-IIIes_ES
dc.subjectChemical screenes_ES
dc.subjectHaploidyes_ES
dc.subjectMitosises_ES
dc.subjectPaclitaxeles_ES
dc.subjectTetraploidyes_ES
dc.titleA Chemical Screen Identifies Compounds Capable of Selecting for Haploidy in Mammalian Cellses_ES
dc.typejournal articlees_ES
dc.type.hasVersionVoRes_ES
dspace.entity.typePublication
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