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Isolation of exophers from cardiomyocyte-reporter mouse strains by fluorescence-activated cell sorting

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dc.contributor.authorNicolás-Ávila, José Ángel
dc.contributor.authorSanchez-Diaz, Maria
dc.contributor.authorHidalgo, Andres
dc.contributor.funderMinisterio de Ciencia e Innovación (España)
dc.contributor.funderFundación La Marató TV3
dc.contributor.funderFundación La Caixa
dc.contributor.funderFondation Leducq
dc.contributor.funderFundación ProCNIC
dc.date.accessioned2021-03-30T12:00:41Z
dc.date.available2021-03-30T12:00:41Z
dc.date.issued2021-01-26
dc.description.abstractCardiac exophers are membrane-bound extracellular vesicles released by cardiomyocytes with varied content and an average diameter of 3.5 μm. Here, we provide a detailed protocol to enable the identification and purification of cardiomyocyte-derived exophers by using fluorescence-activated cell sorting for downstream cellular and molecular analysis. This protocol requires the use of mouse strains expressing fluorescent proteins in cardiomyocytes.es_ES
dc.description.peerreviewedSíes_ES
dc.description.sponsorshipThis study was supported by intramural grant IGP-SO to J.A.-C. and A.H.; and grants 120/C/2015-20153032 from Fundacio´ LaMarato´ de TV3, SAF2015-65607-R and RTI2018-095497-B-I00 from MICINN, HR17_00527 from La Caixa Foundation, and TNE-18CVD04 from the Leducq Foundation to A.H. J.A.N.-A. and M.S.-D. are supported by fellowships SVP- 2014-068595 and PRE2019-08746, respectively. The CNIC is supported by the MICINN and the Pro-CNIC Foundation, and is a SeveroOchoa Center of Excellence (MICINN award SEV-2015-0505).es_ES
dc.format.number1es_ES
dc.format.page100286es_ES
dc.format.volume2es_ES
dc.identifier.citationSTAR Protoc. 2021; 2(1):100286es_ES
dc.identifier.doi10.1016/j.xpro.2020.100286es_ES
dc.identifier.issn2666-1667
dc.identifier.journalSTAR Protocolses_ES
dc.identifier.pubmedID33490991es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/12478
dc.language.isoenges_ES
dc.publisherCell Presses_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/120/C/2015-20153032es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2015-65607-Res_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/RTI2018-095497-B-I00es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/HR17_00527es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SVP- 2014-068595es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/PRE2019-08746es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SEV-2015-0505es_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.xpro.2020.100286es_ES
dc.repisalud.institucionCNICes_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Imagen de la Inflamación Cardiovascular y la Respuesta Inmunees_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.titleIsolation of exophers from cardiomyocyte-reporter mouse strains by fluorescence-activated cell sortinges_ES
dc.typejournal articlees_ES
dc.type.hasVersionVoRes_ES
dspace.entity.typePublication
relation.isAuthorOfPublication95ebb758-8525-4c20-9c24-cfd1455fc6ab
relation.isAuthorOfPublicationacb8e30b-34b9-4718-b517-8d5962f70950
relation.isAuthorOfPublication.latestForDiscovery95ebb758-8525-4c20-9c24-cfd1455fc6ab

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