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Therapeutic Effects of Platelet-Derived Extracellular Vesicles in a Bioengineered Tendon Disease Model

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dc.contributor.authorGraca, Ana L
dc.contributor.authorDomingues, Rui MA
dc.contributor.authorCalejo, Isabel
dc.contributor.authorGomez-Florit, Manuel
dc.contributor.authorGomes, Manuela E
dc.date.accessioned2024-10-04T13:58:02Z
dc.date.available2024-10-04T13:58:02Z
dc.date.issued2022-03
dc.description.abstractTendon injuries represent over 30-50% of musculoskeletal disorders worldwide, yet the available therapies do not provide complete tendon repair/regeneration and full functionality restoring. Extracellular vesicles (EVs), membrane-enclosed nanopresearch articles, have emerged as the next breakthrough in tissue engineering and regenerative medicine to promote endogenous tissue regeneration. Here, we developed a 3D human in vitro model mimicking the signature of pathological tendon and used it to evaluate the influence that different platelet-derived EVs might have in tendon tissue repair mechanisms. For this, different EV populations isolated from platelets, small EVs (sEVs) and medium EVs (mEVs), were added to the culture media of human tendon-derived cells (hTDCs) cultured on isotropic nanofibrous scaffolds. The platelet-derived EVs increased the expression of tenogenic markers, promoted a healthy extracellular matrix (ECM) remodeling, and the synthesis of anti-inflammatory mediators. These findings suggest that platelet EVs provided relevant biochemical cues that potentiated a recovery of hTDCs phenotype from a diseased to a healthy state. Thus, this study opens new perspectives for the translation of platelet-derived EVs as therapeutics.en
dc.description.sponsorshipThis research was funded by the ERC CoG MagTendon grant agreement 772817; EC Twinning project Achilles 810850; Fundacao para a Ciencia e a Tecnologia (FCT) for PhD grant PD/59/2013 and PD/BD/135255/2017, individual contracts CEECIND/01375/2017 (M.G.-F.) and 2020.03410.CEECIND (R.M.A.D), and project PTDC/NAN-MAT/30595/2017.es_ES
dc.format.number6es_ES
dc.format.page2948es_ES
dc.format.volume23es_ES
dc.identifier.citationGraca AL, Domingues RMA, Calejo I, Gomez-Florit M, Gomes ME. Therapeutic Effects of Platelet-Derived Extracellular Vesicles in a Bioengineered Tendon Disease Model. Int J Mol Sci. 2022 Mar;23(6):2948.en
dc.identifier.doi10.3390/ijms23062948
dc.identifier.e-issn1422-0067es_ES
dc.identifier.journalInternational Journal of Molecular Scienceses_ES
dc.identifier.otherhttps://hdl.handle.net/20.500.13003/19872
dc.identifier.pubmedID35328370es_ES
dc.identifier.puiL2015890998
dc.identifier.scopus2-s2.0-85125998564
dc.identifier.urihttps://hdl.handle.net/20.500.12105/23545
dc.identifier.wos775309600001
dc.language.isoengen
dc.publisherMultidisciplinary Digital Publishing Institute (MDPI)
dc.relation.publisherversionhttps://doi.org/10.3390/ijms23062948en
dc.rights.accessRightsopen accessen
dc.rights.licenseAttribution 4.0 International*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectExtracellular vesicles
dc.subjectPlatelets
dc.subjectTendinopathy
dc.subjectTendon-derived cells
dc.subjectIn vitro models
dc.subjectFibers
dc.subjectTissue engineering
dc.subject.decsPlaquetas*
dc.subject.decsEnfermedades Musculoesqueléticas*
dc.subject.decsHumanos*
dc.subject.decsMedicina Regenerativa*
dc.subject.decsVesículas Extracelulares*
dc.subject.decsTendones*
dc.subject.meshTendons*
dc.subject.meshExtracellular Vesicles*
dc.subject.meshMusculoskeletal Diseases*
dc.subject.meshBlood Platelets*
dc.subject.meshHumans*
dc.subject.meshRegenerative Medicine*
dc.titleTherapeutic Effects of Platelet-Derived Extracellular Vesicles in a Bioengineered Tendon Disease Modelen
dc.typeresearch articleen
dspace.entity.typePublication
relation.isPublisherOfPublication30293a55-0e53-431f-ae8c-14ab01127be9
relation.isPublisherOfPublication.latestForDiscovery30293a55-0e53-431f-ae8c-14ab01127be9

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