Publication:
Graph based study of allergen cross-reactivity of plant lipid transfer proteins (LTPs) using microarray in a multicenter study.

Simple item page
dc.contributor.authorPalacín, Arantxa
dc.contributor.authorGómez-Casado, Cristina
dc.contributor.authorRivas, Luis A
dc.contributor.authorAguirre, Jacobo
dc.contributor.authorTordesillas, Leticia
dc.contributor.authorBartra, Joan
dc.contributor.authorCarrillo, Teresa
dc.contributor.authorCuesta-Herranz, Javier
dc.contributor.authorFrutos, Consolación de
dc.contributor.authorGarcía Alvarez-Eire, Genoveva
dc.contributor.authorFernández, Francisco J
dc.contributor.authorGamboa, Pedro
dc.contributor.authorMuñoz, Rosa
dc.contributor.authorSánchez-Monge, Rosa
dc.contributor.authorSirvent, Sofía
dc.contributor.authorTorres, María J
dc.contributor.authorVarela-Losada, Susana
dc.contributor.authorRodríguez, Rosalía
dc.contributor.authorParro, Victor
dc.contributor.authorBlanca, Miguel
dc.contributor.authorSalcedo, Gabriel
dc.contributor.authorDíaz-Perales, Araceli
dc.contributor.authoraffiliation[Palacín,A; Gómez-Casado,C; Tordesillas,L; Sánchez-Monge,R; Díaz-Perales,A] Centro de Biotecnología y Genómica de Plantas (UPM-INIA), Pozuelo de Alarcón, Madrid, Spain. [Rivas,LA; Aguirre,J; Parro,V] Departamento de Evolución Molecular, Centro de Astrobiología (INTA-CSIC), Torrejón de Ardoz, Madrid, Spain. [Bartra,J; Muñoz,R] Unitat d’Alèrgia Servei Pneumologia i Alèrgia Respiratòria Universitat de Barcelona, Institut d’Investigacions Biomèdiques August Pi i Sunyer (IDIBAPS), Centro de Investigaciones Biomédicas en Red de Enfermedades Respiratorias (CIBERES), Barcelona, Spain. [Blanco,C; Frutos,C de] Servicio de Alergia, Hospital Universitario de la Princesa, Instituto de Investigación Sanitaria Princesa (IP), Madrid, Spain. [Carrillo,T] Servicio de Alergología, Hospital Universitario de Gran Canaria Dr. Negrín, Las Palmas de Gran Canaria, Spain. [Cuesta-Herranz,J] Unidad Servicio de Alergia, Fundación Jiménez Díaz, Madrid, Spain. [Fernández,FJ] Allergy Section, General University Hospital, Miguel Hernández University, Elche, Spain. [García Álvarez-Eire,G; Varela-Losada,S] Unidad de Alergología, Complexo Hospitalario, Ourense, Spain. [Torres,MJ; Blanca,M] Research Laboratory, Fundación IMABIS-Carlos Haya Hospital; Hospital Civil, Málaga, Spain. [Gamboa,P] Servicio de Alergia, Hospital de Basurto, Bilbao, Spain. [Sirvent,S; Rodríguez,R] Departamento de Bioquímica y Biología Molecular I, Facultad de Químicas, Universidad Complutense, Madrid, Spain. [Salcedo,G] Departamento de Biotecnología, ETSI Agrónomos, Universidad Politécnica, Madrid, Spain.
dc.date.accessioned2024-01-15T18:16:55Z
dc.date.available2024-01-15T18:16:55Z
dc.date.issued2012-12-14
dc.description.abstractThe study of cross-reactivity in allergy is key to both understanding. the allergic response of many patients and providing them with a rational treatment In the present study, protein microarrays and a co-sensitization graph approach were used in conjunction with an allergen microarray immunoassay. This enabled us to include a wide number of proteins and a large number of patients, and to study sensitization profiles among members of the LTP family. Fourteen LTPs from the most frequent plant food-induced allergies in the geographical area studied were printed into a microarray specifically designed for this research. 212 patients with fruit allergy and 117 food-tolerant pollen allergic subjects were recruited from seven regions of Spain with different pollen profiles, and their sera were tested with allergen microarray. This approach has proven itself to be a good tool to study cross-reactivity between members of LTP family, and could become a useful strategy to analyze other families of allergens.
dc.description.sponsorshipMinisterio de Ciencia e Innovación (projects BIO2009-07050 and SAF2008-04053) and FIS-Thematic Networks, and Co-operative Research Centers: RIRAAF (RD07/0064). C Gómez Casado and L Tordesillas have been supported by training grants from the Spanish Government (FPI and FPU programmes, MEC, respectively). J Aguirre has been supported by the Ministerio de Ciencia e Innovación (project FIS2011-27569) and Comunidad de Madrid, R&D Program of Activities, grant MODELICO-CM S2009ESP-1691.
dc.identifier.doi10.1371/journal.pone.0050799
dc.identifier.e-issn1932-6203es_ES
dc.identifier.journalPloS onees_ES
dc.identifier.otherhttp://hdl.handle.net/10668/1212
dc.identifier.pubmedID23272072es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/17044
dc.language.isoeng
dc.publisherPublic Library of Science (PLOS)
dc.relation.publisherversionhttp://www.plosone.org/article/info%3Adoi%2F10.1371%2Fjournal.pone.0050799es
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAttribution 4.0 International*
dc.rights.urihttps://creativecommons.org/licenses/by/4.0/*
dc.subjectReacciones Cruzadas
dc.subjectEstudios multicéntricos como asunto
dc.subjectHipersensibilidad
dc.subjectAnálisis de micromatrices
dc.subjectInmunoanálisis
dc.subjectEspaña
dc.subject.meshCross Reactions
dc.subject.meshCarrier Proteins
dc.subject.meshPlant Proteins
dc.subject.meshAntigens, Plant
dc.subject.meshMulticenter Studies as Topic
dc.subject.meshHypersensitivity
dc.subject.meshMicroarray Analysis
dc.subject.meshImmunoassay
dc.subject.meshFruit
dc.subject.meshPlants
dc.subject.meshSpain
dc.subject.meshPollen
dc.titleGraph based study of allergen cross-reactivity of plant lipid transfer proteins (LTPs) using microarray in a multicenter study.
dc.typeresearch article
dc.type.hasVersionVoR
dspace.entity.typePublication
relation.isPublisherOfPublicationa2759e3d-0d58-4e8a-9fcd-c6130ee333d1
relation.isPublisherOfPublication.latestForDiscoverya2759e3d-0d58-4e8a-9fcd-c6130ee333d1

Files

Collections

IBIMA-Plataforma BIONAND - Instituto de Investigación Biomédica de Málaga y Plataforma en Nanomedicina (Andalucía)
IDIBAPS - Instituto de Investigaciones Biomédicas August Pi i Sunyer (Cataluña)
IIS-PRINCESA - Instituto de Investigación Sanitaria Hospital Universitario de La Princesa (Madrid)