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Molecular identification of adenoviruses in clinical samples by analyzing a partial hexon genomic region

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dc.contributor.authorCasas Flecha, Inmaculada
dc.contributor.authorAvellón, Ana
dc.contributor.authorMosquera Gutierrez, Maria del Mar
dc.contributor.authorJabado, O
dc.contributor.authorEchevarria, Juan Emilio
dc.contributor.authorCampos, R H
dc.contributor.authorRewers, M
dc.contributor.authorPerez-Breña, Pilar
dc.contributor.authorLipkin, W I
dc.contributor.authorPalacios, G
dc.contributor.funderInstituto de Salud Carlos III
dc.date.accessioned2019-08-14T07:33:13Z
dc.date.available2019-08-14T07:33:13Z
dc.date.issued2005-12
dc.description.abstractHere we present a system for adenovirus detection and genotyping based on PCR amplification and phylogenetic analysis of a conserved hexon gene fragment. The system was validated using 157 sequences (86 previously typed and 71 clinical samples) and correctly identified species and serotype in 100% and 84% of sequences, respectively. Known associations between specific serotypes and clinical syndromes are verified. Possible new associations are described to allow further independent testing.es_ES
dc.description.sponsorshipWe acknowledge technical assistance from Manuela López-Valero in the Respiratory Viruses Laboratory, CNM, ISCIII, Majadahonda, Madrid, Spain. We thank Neil Renwick for his critical reviewing of the manuscript and helpful comments. We also thank the reviewers for their constructive criticism and helpful suggestions on the initial draft. This work was supported by grants from the National Institutes of Health (AI51292, AI55466, AI056118, and U54AI057158-Northeast Biodefense Center), the Ellison Medical Foundation, and ISCIII (MPY 1251/00 and MPY 1018/04).es_ES
dc.format.number12es_ES
dc.format.page6176-82es_ES
dc.format.volume43es_ES
dc.identifier.citationJ Clin Microbiol. 2005 Dec;43(12):6176-82.es_ES
dc.identifier.doi10.1128/JCM.43.12.6176-6182.2005es_ES
dc.identifier.issn0095-1137es_ES
dc.identifier.journalJournal of clinical microbiologyes_ES
dc.identifier.pubmedID16333124es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/8278
dc.language.isoenges_ES
dc.publisherAmerican Society for Microbiology (ASM)
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/AI51292es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/AI55466es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/AI056118es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/U54AI057158es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/MPY1251/00es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/MPY1018/04es_ES
dc.relation.publisherversionhttps://doi.org/10.1128/JCM.43.12.6176-6182.2005es_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject.meshAdenovirus Infections, Humanes_ES
dc.subject.meshAdenoviruses, Humanes_ES
dc.subject.meshAdolescentes_ES
dc.subject.meshAdultes_ES
dc.subject.meshCapsid Proteinses_ES
dc.subject.meshChildes_ES
dc.subject.meshChild, Preschooles_ES
dc.subject.meshDNA, Virales_ES
dc.subject.meshGenome, Virales_ES
dc.subject.meshHumanses_ES
dc.subject.meshInfantes_ES
dc.subject.meshInfant, Newbornes_ES
dc.subject.meshMiddle Agedes_ES
dc.subject.meshMolecular Sequence Dataes_ES
dc.subject.meshPhylogenyes_ES
dc.subject.meshPolymerase Chain Reactiones_ES
dc.subject.meshSerotypinges_ES
dc.subject.meshSequence Analysis, DNAes_ES
dc.titleMolecular identification of adenoviruses in clinical samples by analyzing a partial hexon genomic regiones_ES
dc.typeresearch articlees_ES
dc.type.hasVersionAMes_ES
dspace.entity.typePublication
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