Publication:
Intensity distribution segmentation in ultrafast Doppler combined with scanning laser confocal microscopy for assessing vascular changes associated with ageing in murine hippocampi

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dc.contributor.authorAnzibar Fialho, Maximiliano
dc.contributor.authorVázquez Alberdi, Lucia
dc.contributor.authorMartínez, Mariana
dc.contributor.authorCalero, Miguel
dc.contributor.authorBaranger, Jerome
dc.contributor.authorTanter, Mickael
dc.contributor.authorDamián, Juan Pablo
dc.contributor.authorNegreira, Carlos
dc.contributor.authorRubido, Nicolás
dc.contributor.authorKun, Alejandra
dc.contributor.authorBrum, Javier
dc.contributor.funderAgencia Nacional de Investigación e Innovación (Uruguay)es_ES
dc.date.accessioned2022-08-03T07:50:53Z
dc.date.available2022-08-03T07:50:53Z
dc.date.issued2022
dc.descriptionPublisher Correction: Intensity distribution segmentation in ultrafast Doppler combined with scanning laser confocal microscopy for assessing vascular changes associated with ageing in murine hippocampi PMID: 35538217 https://doi.org/10.1038/s41598-022-11822-4es_ES
dc.description.abstractThe hippocampus plays an important role in learning and memory, requiring high-neuronal oxygenation. Understanding the relationship between blood flow and vascular structure-and how it changes with ageing-is physiologically and anatomically relevant. Ultrafast Doppler ([Formula: see text]Doppler) and scanning laser confocal microscopy (SLCM) are powerful imaging modalities that can measure in vivo cerebral blood volume (CBV) and post mortem vascular structure, respectively. Here, we apply both imaging modalities to a cross-sectional and longitudinal study of hippocampi vasculature in wild-type mice brains. We introduce a segmentation of CBV distribution obtained from [Formula: see text]Doppler and show that this mice-independent and mesoscopic measurement is correlated with vessel volume fraction (VVF) distribution obtained from SLCM-e.g., high CBV relates to specific vessel locations with large VVF. Moreover, we find significant changes in CBV distribution and vasculature due to ageing (5 vs. 21 month-old mice), highlighting the sensitivity of our approach. Overall, we are able to associate CBV with vascular structure-and track its longitudinal changes-at the artery-vein, venules, arteriole, and capillary levels. We believe that this combined approach can be a powerful tool for studying other acute (e.g., brain injuries), progressive (e.g., neurodegeneration) or induced pathological changes.es_ES
dc.description.peerreviewedSíes_ES
dc.description.sponsorshipThis work was mainly funded by the Agencia Nacional de Investigación e Innovación (ANII), grant FCE_1_2019_1_155539. The authors also thank the support of PEDECIBA, CSIC-UdelaR and the Institut Franco—Uruguayen de Physique (IFUP), LIA-CNRS-UdelaR. J.P.D., C.N., N.R., A.K. and J.Br. thank SNI-ANII. M.A.F. thanks the support of ANII through POS_NAC_M_2020_1_164127 scholarship. M.M. thanks the support of ANII through POS_FCE_2020_1_1009181 scholarship. N.R. thanks the support of CSIC I+D group grant CSIC2018—FID 13—Grupo ID 722.es_ES
dc.format.number1es_ES
dc.format.page6784es_ES
dc.format.volume12es_ES
dc.identifier.citationSci Rep. 2022 Apr 26;12(1):6784.es_ES
dc.identifier.doi10.1038/s41598-022-10457-9es_ES
dc.identifier.e-issn2045-2322es_ES
dc.identifier.journalScientific Reportses_ES
dc.identifier.pubmedID35473942es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/14814
dc.language.isoenges_ES
dc.publisherNature Publishing Groupes_ES
dc.relation.publisherversionhttps://doi.org/10.1038/s41598-022-10457-9es_ES
dc.repisalud.centroISCIII::Unidad Funcional de Investigación de Enfermedades Crónicas (UFIEC)es_ES
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subjectAgeinges_ES
dc.subjectConfocal microscopyes_ES
dc.subjectImaging techniqueses_ES
dc.subjectNeural ageinges_ES
dc.subjectUltrasoundes_ES
dc.subject.meshAginges_ES
dc.subject.meshHippocampuses_ES
dc.subject.meshAnimalses_ES
dc.subject.meshCross-Sectional Studieses_ES
dc.subject.meshLaserses_ES
dc.subject.meshLongitudinal Studieses_ES
dc.subject.meshMicees_ES
dc.subject.meshMicroscopy, Confocales_ES
dc.titleIntensity distribution segmentation in ultrafast Doppler combined with scanning laser confocal microscopy for assessing vascular changes associated with ageing in murine hippocampies_ES
dc.typejournal articlees_ES
dc.type.hasVersionVoRes_ES
dspace.entity.typePublication
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