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Impact of human-associated Escherichia coli clonal groups in Antarctic pinnipeds: presence of ST73, ST95, ST141 and ST131

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dc.contributor.authorMora, Azucena
dc.contributor.authorGarcía-Peña, Francisco Javier
dc.contributor.authorAlonso, María Pilar
dc.contributor.authorPedraza-Diaz, Susana
dc.contributor.authorOrtega-Mora, Luis Miguel
dc.contributor.authorGarcia-Parraga, Daniel
dc.contributor.authorLópez, Cecilia
dc.contributor.authorViso, Susana
dc.contributor.authorDahbi, Ghizlane
dc.contributor.authorMarzoa, Juan
dc.contributor.authorSergeant, Martin J
dc.contributor.authorGarcía, Vanesa
dc.contributor.authorBlanco, Jorge
dc.contributor.funderMinisterio de Ciencia e Innovación (España)es_ES
dc.contributor.funderMinisterio de Economía y Competitividad (España)es_ES
dc.contributor.funderUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)es_ES
dc.contributor.funderAgencia Estatal de Investigación (España)es_ES
dc.contributor.funderInstituto de Salud Carlos IIIes_ES
dc.contributor.funderXunta de Galicia (España)es_ES
dc.date.accessioned2020-02-28T07:56:05Z
dc.date.available2020-02-28T07:56:05Z
dc.date.issued2018
dc.description.abstractThere is growing concern about the spreading of human microorganisms in relatively untouched ecosystems such as the Antarctic region. For this reason, three pinniped species (Leptonychotes weddellii, Mirounga leonina and Arctocephalus gazella) from the west coast of the Antartic Peninsula were analysed for the presence of Escherichia spp. with the recovery of 158 E. coli and three E. albertii isolates. From those, 23 harboured different eae variants (α1, β1, β2, ε1, θ1, κ, ο), including a bfpA-positive isolate (O49:H10-A-ST206, eae-k) classified as typical enteropathogenic E. coli. Noteworthy, 62 of the 158 E. coli isolates (39.2%) exhibited the ExPEC status and 27 (17.1%) belonged to sequence types (ST) frequently occurring among urinary/bacteremia ExPEC clones: ST12, ST73, ST95, ST131 and ST141. We found similarities >85% within the PFGE-macrorrestriction profiles of pinniped and human clinic O2:H6-B2-ST141 and O16:H5/O25b:H4-B2-ST131 isolates. The in silico analysis of ST131 Cplx genomes from the three pinnipeds (five O25:H4-ST131/PST43-fimH22-virotype D; one O16:H5-ST131/PST506-fimH41; one O25:H4-ST6252/PST9-fimH22-virotype D1) identified IncF and IncI1 plasmids and revealed high core-genome similarities between pinniped and human isolates (H22 and H41 subclones). This is the first study to demonstrate the worrisome presence of human-associated E. coli clonal groups, including ST131, in Antarctic pinnipeds.es_ES
dc.description.peerreviewedSíes_ES
dc.description.sponsorshipThe sampling was funded by the Spanish Ministry of Science and Innovation (CGL-2005-25073-E/ANT and CTM2008-00570) and the Sea World & Bush Gardens Conservation Fund. Work at USC-LREC was supported by projects AGL2013-47852-R from the Ministerio de Economía y Competitividad (MINECO, Spain) and Fondo Europeo de Desarrollo Regional (FEDER); AGL2016-79343-R from the Agencia Estatal de Investigación (AEI, Spain) and FEDER; PI16/01477 from Plan Estatal de I + D + I 2013-2016, Instituto de Salud Carlos III (ISCIII), Subdirección General de Evaluación y Fomento de la Investigación, and FEDER; CN2012/303 and ED431C 2017/57 from the Consellería de Cultura, Educación e Ordenación Universitaria, (Xunta de Galicia) and FEDER. We would like to thank the military personnel at the Spanish Antarctic Base “Gabriel de Castilla” for their help and assistance and the Marine Technology Unit (CSIC), the Spanish Navy’s Oceanographic Research Ship “Las Palmas” and the Unidad de Tecnología Marítima (UTM, CSIC) for logistics and transport. We also express our gratitude to J. Castro-Urda, F.T. García-Moreno, C. Rengifo-Herrera, S. Rojo-Montejo, I. Ferre, V. Navarro, M. Gómez-Bautista, T. Alvaro-Alvarez and J. Coello-Pérez for their invaluable help in the sample collection. A. Mora acknowledges the Ministerio de Educación, Cultura y Deporte (Spain) for the mobility grant for teachers and researchers from the Programa Estatal de Promoción del Talento y su Empleabilidad, Plan Estatal de Investigación Científica y Técnica y de Innovación 2013–2016.es_ES
dc.format.number1es_ES
dc.format.page4678es_ES
dc.format.volume8es_ES
dc.identifier.citationSci Rep. 2018 Mar 16;8(1):4678.es_ES
dc.identifier.doi10.1038/s41598-018-22943-0es_ES
dc.identifier.e-issn2045-2322es_ES
dc.identifier.issn2045-2322es_ES
dc.identifier.journalScientific reportses_ES
dc.identifier.pubmedID29549276es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/9151
dc.language.isoenges_ES
dc.publisherNature Publishing Groupes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/CGL-2005-25073-E/ANTes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/CTM2008-00570es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/AGL2013-47852-Res_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/AGL2016-79343-Res_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/PI16/01477es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/CN2012/303es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/ED431C 2017/57es_ES
dc.relation.publisherversionhttps://doi.org/10.1038/s41598-018-22943-0es_ES
dc.repisalud.centroISCIII::Centro Nacional de Sanidad Ambientales_ES
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.meshAnimalses_ES
dc.subject.meshAntarctic Regionses_ES
dc.subject.meshBacterial Typing Techniqueses_ES
dc.subject.meshCaniformiaes_ES
dc.subject.meshDNA, Bacteriales_ES
dc.subject.meshEcosystemes_ES
dc.subject.meshEscherichia colies_ES
dc.subject.meshEscherichia coli Infectionses_ES
dc.subject.meshHumanses_ES
dc.subject.meshMolecular Epidemiologyes_ES
dc.subject.meshMolecular Typinges_ES
dc.subject.meshPhylogenyes_ES
dc.titleImpact of human-associated Escherichia coli clonal groups in Antarctic pinnipeds: presence of ST73, ST95, ST141 and ST131es_ES
dc.typejournal articlees_ES
dc.type.hasVersionVoRes_ES
dspace.entity.typePublication
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