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Substitutions at methionine 220 in the 14alpha-sterol demethylase (Cyp51A) of Aspergillus fumigatus are responsible for resistance in vitro to azole antifungal drugs

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dc.contributor.authorMellado, Emilia
dc.contributor.authorGarcia-Effron, Guillermo
dc.contributor.authorAlcazar-Fuoli, Laura
dc.contributor.authorCuenca-Estrella, Manuel
dc.contributor.authorRodriguez-Tudela, Juan Luis
dc.contributor.funderMinisterio de Ciencia y Tecnología (España)
dc.contributor.funderInstituto de Salud Carlos III
dc.date.accessioned2019-08-14T06:17:24Z
dc.date.available2019-08-14T06:17:24Z
dc.date.issued2004-07
dc.description.abstractFive clinical isolates of Aspergillus fumigatus that exhibited similar patterns of reduced susceptibility to itraconazole and other triazole drugs were analyzed. Sequence analysis of genes (cyp51A and cyp51B) encoding the 14alpha-sterol demethylases revealed that all five strains harbored mutations in cyp51A resulting in the replacement of methionine at residue 220 by valine, lysine, or threonine. When the mutated cyp51A genes were introduced into an A. fumigatus wild-type strain, the transformants exhibited reduced susceptibility to all triazole agents, confirming that the mutations were responsible for the resistance phenotype.es_ES
dc.description.sponsorshipThis work was supported in part by grants MPY1120/03 from Instituto de Salud Carlos III and SAF2002-02089 from the Ministry of Science and Technology. E. Mellado held a Ramón y Cajal contract from Ministry of Science and Technology. L. Alcazar-Fuoli has a predoctoral fellowship from Instituto de Salud Carlos III.es_ES
dc.format.number7es_ES
dc.format.page2747-50es_ES
dc.format.volume48es_ES
dc.identifier.citationAntimicrob Agents Chemother. 2004 Jul;48(7):2747-50.es_ES
dc.identifier.doi10.1128/AAC.48.7.2747-2750.2004es_ES
dc.identifier.issn0066-4804es_ES
dc.identifier.journalAntimicrobial agents and chemotherapyes_ES
dc.identifier.pubmedID15215142es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/8260
dc.language.isoenges_ES
dc.publisherAmerican Society for Microbiology (ASM)
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2002-02089es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/MPY1120/03es_ES
dc.relation.publisherversionhttps://doi.org/10.1128/AAC.48.7.2747-2750.2004es_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAtribución-NoComercial-CompartirIgual 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-sa/4.0/*
dc.subject.meshAlleleses_ES
dc.subject.meshAmino Acid Sequencees_ES
dc.subject.meshAmino Acid Substitutiones_ES
dc.subject.meshAntifungal Agentses_ES
dc.subject.meshAspergillus fumigatuses_ES
dc.subject.meshAzoleses_ES
dc.subject.meshCytochrome P-450 Enzyme Systemes_ES
dc.subject.meshDrug Resistance, Fungales_ES
dc.subject.meshFungal Proteinses_ES
dc.subject.meshMethioninees_ES
dc.subject.meshMicrobial Sensitivity Testses_ES
dc.subject.meshMolecular Sequence Dataes_ES
dc.subject.meshMutationes_ES
dc.subject.meshReverse Transcriptase Polymerase Chain Reactiones_ES
dc.titleSubstitutions at methionine 220 in the 14alpha-sterol demethylase (Cyp51A) of Aspergillus fumigatus are responsible for resistance in vitro to azole antifungal drugses_ES
dc.typeresearch articlees_ES
dc.type.hasVersionAMes_ES
dspace.entity.typePublication
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