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Molecular epidemiology, antimicrobial susceptibilities and resistance mechanisms of Streptococcus pyogenes isolates resistant to erythromycin and tetracycline in Spain (1994-2006)

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dc.contributor.authorRubio-Lopez, Virginia
dc.contributor.authorValdezate, Sylvia
dc.contributor.authorÁlvarez, David
dc.contributor.authorVillalon-Panzano, Pilar
dc.contributor.authorMedina-Pascual, Maria Jose
dc.contributor.authorSalcedo, Celia
dc.contributor.authorSaez, Juan Antonio
dc.contributor.funderInstituto de Salud Carlos III
dc.contributor.funderMinisterio de Ciencia e Innovación (España)
dc.date.accessioned2019-01-30T16:56:56Z
dc.date.available2019-01-30T16:56:56Z
dc.date.issued2012-09-21
dc.description.abstractBACKGROUND: Group A Streptococcus (GAS) causes human diseases ranging in severity from uncomplicated pharyngitis to life-threatening necrotizing fasciitis and shows high rates of macrolide resistance in several countries. Our goal is to identify antimicrobial resistance in Spanish GAS isolates collected between 1994 and 2006 and to determine the molecular epidemiology (emm/T typing and PFGE) and resistance mechanisms of those resistant to erythromycin and tetracycline. RESULTS: Two hundred ninety-five out of 898 isolates (32.8%) were erythromycin resistant, with the predominance of emm4T4, emm75T25, and emm28T28, accounting the 67.1% of the 21 emm/T types. Spread of emm4T4, emm75T25 and emm28T28 resistant clones caused high rates of macrolide resistance. The distribution of the phenotypes was M (76.9%), cMLSB (20.3%), iMLSB (2.7%) with the involvement of the erythromycin resistance genes mef(A) (89.5%), msr(D) (81.7%), erm(B) (37.3%) and erm(A) (35.9%).Sixty-one isolates were tetracycline resistant, with the main representation of the emm77T28 among 20 emm/T types. To note, the combination of tet(M) and tet(O) tetracycline resistance genes were similar to tet(M) alone reaching values close to 40%. Resistance to both antibiotics was detected in 19 isolates of 7 emm/T types, being emm11T11 and the cMLSB phenotype the most frequent ones. erm(B) and tet(M) were present in almost all the strains, while erm(A), mef(A), msr(D) and tet(O) appeared in less than half of them. CONCLUSIONS: Spanish GAS were highly resistant to macrolides meanwhile showed minor resistance rate to tetracycline. A remarkable correlation between antimicrobial resistance and emm/T type was noticed. Clonal spread of emm4T4, emm75T25 and emm28T28 was the main responsable for macrolide resistance where as that emm77T28 clones were it to tetraclycline resistance. A wide variety of macrolide resistance genes were responsible for three macrolide resistance phenotypes.es_ES
dc.description.peerreviewedSíes_ES
dc.description.sponsorshipThis research was funded by an intramural predoctoral fellowship from the Carlos III Health Institute (grant number 05/0030) and the Spanish Ministry of Science and Innovation.es_ES
dc.format.number1es_ES
dc.format.page215es_ES
dc.format.volume12es_ES
dc.identifier.citationBMC Microbiol. 2012 Sep 21;12:215.es_ES
dc.identifier.doi10.1186/1471-2180-12-215es_ES
dc.identifier.e-issn1471-2180es_ES
dc.identifier.issn1471-2180es_ES
dc.identifier.journalBMC microbiologyes_ES
dc.identifier.pubmedID22998619es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/7026
dc.language.isoenges_ES
dc.publisherBioMed Central (BMC)es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/05/0030es_ES
dc.relation.publisherversionhttps://doi.org/10.1186/1471-2180-12-215es_ES
dc.repisalud.centroISCIII::Centro Nacional de Microbiologíaes_ES
dc.repisalud.institucionISCIIIes_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.meshAnti-Bacterial Agentses_ES
dc.subject.meshAntigens, Bacteriales_ES
dc.subject.meshBacterial Outer Membrane Proteinses_ES
dc.subject.meshCarrier Proteinses_ES
dc.subject.meshCluster Analysises_ES
dc.subject.meshDNA, Bacteriales_ES
dc.subject.meshElectrophoresis, Gel, Pulsed-Fieldes_ES
dc.subject.meshErythromycines_ES
dc.subject.meshHumanses_ES
dc.subject.meshMicrobial Sensitivity Testses_ES
dc.subject.meshMolecular Epidemiologyes_ES
dc.subject.meshMolecular Typinges_ES
dc.subject.meshSpaines_ES
dc.subject.meshStreptococcal Infectionses_ES
dc.subject.meshStreptococcus pyogeneses_ES
dc.subject.meshTetracyclinees_ES
dc.subject.meshDrug Resistance, Bacteriales_ES
dc.titleMolecular epidemiology, antimicrobial susceptibilities and resistance mechanisms of Streptococcus pyogenes isolates resistant to erythromycin and tetracycline in Spain (1994-2006)es_ES
dc.typejournal articlees_ES
dc.type.hasVersionVoRes_ES
dspace.entity.typePublication
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