Publication:
Flow Cytometry Has a Significant Impact on the Cellular Metabolome

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dc.contributor.authorBinek, Aleksandra
dc.contributor.authorRojo, David
dc.contributor.authorGodzien, Joanna
dc.contributor.authorRupérez, Francisco Javier
dc.contributor.authorNunez, Vanessa
dc.contributor.authorJorge, Inmaculada
dc.contributor.authorRicote, Mercedes
dc.contributor.authorVazquez, Jesus
dc.contributor.authorBarbas, Coral
dc.contributor.funderMinisterio de Economía y Competitividad (España)
dc.contributor.funderInstituto de Salud Carlos III
dc.contributor.funderFundación La Marató TV3
dc.contributor.funderUnión Europea. Fondo Europeo de Desarrollo Regional (FEDER/ERDF)
dc.contributor.funderUnión Europea. Comisión Europea
dc.contributor.funderFundación ProCNIC
dc.date.accessioned2020-05-08T07:16:29Z
dc.date.available2020-05-08T07:16:29Z
dc.date.issued2019-01
dc.description.abstractThe characterization of specialized cell subpopulations in a heterogeneous tissue is essential for understanding organ function in health and disease. A popular method of cell isolation is fluorescence-activated cell sorting (FACS) based on probes that bind surface or intracellular markers. In this study, we analyze the impact of FACS on the cell metabolome of mouse peritoneal macrophages. Compared with directly pelleted macrophages, FACS-treated cells had an altered content of metabolites related to the plasma membrane, activating a mechanosensory signaling cascade causing inflammation-like stress. The procedure also triggered alterations related to energy consumption and cell damage. The observed changes mostly derive from the physical impact on cells during their passage through the instrument. These findings provide evidence of FACS-induced biochemical changes, which should be taken into account in the design of robust metabolic assays of cells separated by flow cytometry.es_ES
dc.description.peerreviewedSíes_ES
dc.description.sponsorshipFJ.R., J.G., and D.R. acknowledge funding from the Ministerio de Economia y Competitividad (CTQ2014-55279-R). This study was also supported by Ministerio de Economia y Competitividad grant BIO2015-67580-P through the Carlos III Institute of Health (ISCIII) and the Fundacion La Marato TV3 to J.V and to M.R (201605-30-31-32). J.V. laboratory is a member of Proteored, PRB3 and is supported by grant PT17/0019, of the PE I+D+i 2013-2016, funded by ISCIII and European Regional Development Fund (ERDF). M.R. received grants from the Ministerio de Economia y Competitividad (SAF2015-64287R, SAF2017-90604-REDT). J.V and M.R received funding from the People Programme (Marie Curie Actions) of the European Union Seventh Framework Programme (FP7/2007-2013) under REA grant agreement no 608027 (CardioNext Initial Training Networks project). A.B. is a FP7-PEOPLE-2013-ITN-Cardionext fellow. The CNIC is supported by the Ministerio de Ciencia, Innovacion y Universidades (MCNU) and the Pro-CNIC Foundation, and is a Severo Ochoa Center of Excellence (SEV-2015-0505).es_ES
dc.format.number1es_ES
dc.format.page169-181es_ES
dc.format.volume18es_ES
dc.identifier.citationJ Proteome Res. 2019; 18(1):16981es_ES
dc.identifier.doi10.1021/acs.jproteome.8b00472es_ES
dc.identifier.e-issn1535-3907es_ES
dc.identifier.issn1535-3893es_ES
dc.identifier.journalJournal of proteome researches_ES
dc.identifier.pubmedID30362351es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/9968
dc.language.isoenges_ES
dc.publisherAmerican Chemical Society (ACS)es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SEV-2015-0505es_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/CTQ2014-55279-Res_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2015-64287Res_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/SAF2017-90604-REDTes_ES
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/FP7/608027es_ES
dc.relation.publisherversionhttps://doi.org/10.1021/acs.jproteome.8b00472es_ES
dc.repisalud.institucionCNICes_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Proteómica cardiovasculares_ES
dc.repisalud.orgCNICCNIC::Grupos de investigación::Señalización de los Receptores Nucleareses_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAttribution-NonCommercial-NoDerivatives 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/*
dc.subjectCE−MSes_ES
dc.subjectGC−MSes_ES
dc.subjectLC−MSes_ES
dc.subjectFluorescence activated cell sorting (FACS)es_ES
dc.subjectMetabolome profilees_ES
dc.subjectMetabolomicses_ES
dc.subjectMultiplatform analysises_ES
dc.subjectSorted cellses_ES
dc.subjectSortinges_ES
dc.subject.meshAnimalses_ES
dc.subject.meshCells, Culturedes_ES
dc.subject.meshFlow Cytometryes_ES
dc.subject.meshMacrophages, Peritoneales_ES
dc.subject.meshMicees_ES
dc.subject.meshResearch Designes_ES
dc.subject.meshCell Separationes_ES
dc.subject.meshMetabolomees_ES
dc.titleFlow Cytometry Has a Significant Impact on the Cellular Metabolomees_ES
dc.typejournal articlees_ES
dc.type.hasVersionAMes_ES
dspace.entity.typePublication
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relation.isAuthorOfPublication.latestForDiscovery1ade9fd4-6ff0-41d9-8edb-9746d704b6ee

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