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Generation and characterization of a human iPSC cell line expressing inducible Cas9 in the "safe harbor" AAVS1 locus

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dc.contributor.authorCastaño, Julio
dc.contributor.authorBueno, Clara
dc.contributor.authorJiménez-Delgado, Senda
dc.contributor.authorRoca-Ho, Heleia
dc.contributor.authorFraga, Mario F
dc.contributor.authorFernandez, Agustín F
dc.contributor.authorNakanishi, Mahito
dc.contributor.authorTorres-Ruiz Raul, Raúl
dc.contributor.authorRodriguez Perales, Sandra
dc.contributor.authorMenéndez, Pablo
dc.date.accessioned2019-04-02T11:45:11Z
dc.date.available2019-04-02T11:45:11Z
dc.date.issued2017
dc.description.abstractWe report the generation-characterization of a fetal liver (FL) B-cell progenitor (BCP)-derived human induced pluripotent stem cell (hiPSC) line CRISPR/Cas9-edited to carry/express a single copy of doxycycline-inducible Cas9 gene in the "safe locus" AAVS1 (iCas9-FL-BCP-hiPSC). Gene-edited iPSCs remained pluripotent after CRISPR/Cas9 genome-edition. Correct genomic integration of a unique copy of Cas9 was confirmed by PCR and Southern blot. Cas9 was robustly and specifically expressed on doxycycline exposure. T7-endonuclease assay demonstrated that iCas9 induces robust gene-edition when gRNAs against hematopoietic transcription factors were tested. This iCas9-FL-BCP-hiPSC will facilitate gene-editing approaches for studies on developmental biology, drug screening and disease modeling.es_ES
dc.description.peerreviewedSíes_ES
dc.format.page137-140es_ES
dc.format.volume21es_ES
dc.identifier.citationStem Cell Res. 2017;21:137-140es_ES
dc.identifier.doi10.1016/j.scr.2017.04.011es_ES
dc.identifier.e-issn1876-7753es_ES
dc.identifier.issn18735061es_ES
dc.identifier.journalStem cell researches_ES
dc.identifier.pubmedID28677529es_ES
dc.identifier.urihttp://hdl.handle.net/20.500.12105/7430
dc.language.isoenges_ES
dc.relation.publisherversionhttps://doi.org/10.1016/j.scr.2017.04.011.es_ES
dc.repisalud.institucionCNIOes_ES
dc.repisalud.orgCNIOCNIO::Unidades técnicas::Unidad de Citogenética Moleculares_ES
dc.rights.accessRightsopen accesses_ES
dc.rights.licenseAtribución 4.0 Internacional*
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/*
dc.subject.meshB-Lymphocyteses_ES
dc.subject.meshBacterial Proteinses_ES
dc.subject.meshCRISPR-Associated Protein 9es_ES
dc.subject.meshCRISPR-Cas Systemses_ES
dc.subject.meshEndonucleaseses_ES
dc.subject.meshGene Editinges_ES
dc.subject.meshHumanses_ES
dc.subject.meshInduced Pluripotent Stem Cellses_ES
dc.subject.meshDependoviruses_ES
dc.subject.meshGene Expressiones_ES
dc.subject.meshGenetic Locies_ES
dc.titleGeneration and characterization of a human iPSC cell line expressing inducible Cas9 in the "safe harbor" AAVS1 locuses_ES
dc.typejournal articlees_ES
dc.type.hasVersionVoRes_ES
dspace.entity.typePublication
relation.isAuthorOfPublicationcac6c6e2-06a9-4548-b216-3d7d32ed6b6e
relation.isAuthorOfPublication.latestForDiscoverycac6c6e2-06a9-4548-b216-3d7d32ed6b6e

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