Garcia, SBernad, AMartín, M CCigudosa, J CGarcia-Castro, Javierde la Fuente, R2019-07-192019-07-192010-05-15Exp Cell Res. 2010 May 15;316(9):1648-50.00144827http://hdl.handle.net/20.500.12105/7965Spontaneous in vitro transformation of human primary cells was, and continues to be, a scarcely described phenomenon. Only the description of the generation of the HaCAT cell line [1] is a canonical example, worldwide accepted. More recent examples included the emergence of tumorogenic populations upon in vitro culture of fetal human mesenchymal stem cells (hMSC), induced by GM-CSF and IL-4, [2] and bone marrow hMSC [3]. Other examples have also been reported after very long-term in vitro culture of telomerized hMSCTerT [4]. In this scenario, our previous results [5-7] were only a new observation to be added to this list. Only very recently similar results have been published [8], reporting spontaneous malignant transformation in 46 % of bone marrow–derived hMSC long-term cultures (5–106 weeks). However, other authors reported opposite results.engAMhttp://creativecommons.org/licenses/by-nc-nd/4.0/HumansIn Vitro TechniquesMesenchymal Stem CellsCell Transformation, NeoplasticAttribution-NonCommercial-NoDerivatives 4.0 Internacional2017196331691648-5010.1016/j.yexcr.2010.02.0161090-2422Experimental cell researchopen access