Gutierrez-Vazquez, CristinaRodriguez-Galan, AnaFernandez-Alfara, MarcosMittelbrunn, MariaSanchez-Cabo, FatimaJorge Martinez-Herrera, DannysRamirez-Huesca, MartaPascual-Montano, AlbertoSanchez-Madrid, Francisco2017-10-202017-10-202017Sci Rep. 2017; 7(1):35082045-2322http://hdl.handle.net/20.500.12105/5111microRNAs (miRNAs) are tightly regulated during T lymphocyte activation to enable the establishment of precise immune responses. Here, we analyzed the changes of the miRNA profiles of T cells in response to activation by cognate interaction with dendritic cells. We also studied mRNA targets common to miRNAs regulated in T cell activation. pik3r1 gene, which encodes the regulatory subunits of PI3K p50, p55 and p85, was identified as target of miRNAs upregulated after T cell activation. Using 3'UTR luciferase reporter-based and biochemical assays, we showed the inhibitory relationship between miR-132-3p upregulation and expression of the pik3r1 gene. Our results indicate that specific miRNAs whose expression is modulated during T cell activation might regulate PI3K signaling in T cells.engVoRMESSENGER-RNAMICRORNAEXPRESSIONDIFFERENTIATIONPI3KPREDICTIONP85-ALPHADUPLEXESDICERAtribución 4.0 Internacional286156447https://doi.org/10.1038/s41598-017-03689-7Scientific Reportopen access