Del Monte-Monge, AlbertoMartos-Folgado, InmaculadaRodríguez-Ronchel, AnaRamiro, Almudena R2023-07-172023-07-172023-04-30STAR Protoc. 2023 Apr 30;4(2):102274http://hdl.handle.net/20.500.12105/16278The antibody immune response plays a critical role in atherosclerosis. Here, we present a protocol for assessing the impact of an antigen-specific germinal center antibody response on atherosclerosis development, using a pro-atherogenic mouse model deficient for the production of germinal-center-derived antibodies. We describe steps for bone marrow transfer from donor mice into irradiated recipient mice. We then detail immunization of mouse chimeras with atheroprotective malondialdehyde low-density lipoprotein during high-fat diet feeding and atherosclerosis burden analysis. For complete details on the use and execution of this protocol, please refer to Martos-Folgado et al. (2022).1.engVoRhttp://creativecommons.org/licenses/by-nc-nd/4.0/Attribution-NonCommercial-NoDerivatives 4.0 Internacional371264444210227410.1016/j.xpro.2023.1022742666-1667STAR protocolsopen access