Quero-Delgado, MartaCodina Márquez, HelenaGómez, RafaelTerán, Francisco JoséMuñoz-Fernández, M AJiménez, José LuisResino, SalvadorSepulveda-Crespo, DanielMartinez, Isidoro2026-06-152026-06-152025-07-29Quero-Delgado, M., Codina, H., Gómez, R., Terán, F.J., Muñoz-Fernández, M.A., Jiménez, J.L., Resino, S., Sepúlveda-Crespo, D. and Martínez, I. (2026), Advances in Nanotechnology-Enabled Optical Biosensors for Dengue Virus Detection: A Systematic Review. Medicinal Research Reviews, 46: 70-111. https://doi.org/10.1002/med.70006https://hdl.handle.net/20.500.12105/27534The dramatic surge in dengue cases in early 2024, endangering half the global population, urgently necessitates faster diagnostic methods. Nanotechnology-enabled optical biosensors offer a promising avenue, leveraging nanomaterial properties for highly sensitive detection of dengue virus (DENV), potentially surpassing conventional techniques in terms of simplicity, speed, and cost-effectiveness. This systematic review analyzes recent advancements in these biosensors for DENV diagnosis. Following PRISMA 2020 guidelines, we systematically searched PubMed, Embase, Scopus, Web of Science, and Cochrane Library databases (2010-June 1, 2025; OSF: https://osf.io/3gmey/). The methodological quality of the 98 included studies was assessed using a modified CASP checklist. Diverse nanotechnology-based optical biosensors were identified. SPR (36.7%) was the most common transducer, followed by fluorescence (24.5%), colorimetry (15.3%), and SERS (8.2%). Gold-based nanomaterials (35.7%) were most frequently employed, with silver nanomaterials (15.3%), quantum dots (15.3%), and graphene-based materials (15.3%) also showing promise. DENV NS1 protein was the primary target analyte (21.4%). Importantly, almost half of the studies (44.9%) used clinically relevant human samples. While many optical biosensors show promise, challenges hinder their demonstration of the true potential for point-of-care use in their current format; however, they offer high specificity and faster results, laying a strong foundation for cost-effective clinical diagnostics. Nanotechnology-driven optical biosensors offer a transformative approach for DENV detection. Advances in computational design and green synthesis of novel nanomaterials are key to addressing stability and field-deployment challenges. These innovations are crucial for developing robust, sensitive, and user-friendly tools to manage dengue and improve patient outcomes globally.engAMhttp://creativecommons.org/licenses/by-nc-nd/4.0/Dengue virusDiagnosticsNanomaterialsOptical biosensorPoint‐of‐careBiosensing TechniquesDengue VirusDengueHumansNanostructuresNanotechnologyAttribution-NonCommercial-NoDerivatives 4.0 International4073418646170-11110.1002/med.70006Medicinal Research Reviewsembargoed access