Santos-Lozano, AlejandroValenzuela, Pedro LLlavero, FranciscoLista, SimoneCarrera-Bastos, PedroHampel, HaraldPareja-Galeano, HeliosGálvez, Beatriz GLopez, Juan AntonioVazquez, JesusEmanuele, EnzoZugaza, José LLucia, Alejandro2020-03-092020-03-092020-02Aging (Albany NY). 2020; 12(4):3502-35151945-4589http://hdl.handle.net/20.500.12105/9247Healthy aging depends on a complex gene-environment network that is ultimately reflected in the expression of different proteins. We aimed to perform a comparative analysis of the plasma proteome of healthy centenarians (n=9, 5 women, age range 100-103 years) with a notably preserved ambulatory capacity (as a paradigm of 'successful' aging), and control individuals who died from a major age-related disease before the expected life expectancy (n=9, 5 women, age range: 67-81 years), and while having impaired ambulatory capacity (as a paradigm of 'unsuccessful' aging). We found that the expression of 49 proteins and 86 pathways differed between the two groups. Overall, healthy centenarians presented with distinct expression of proteins/pathways that reflect a healthy immune function, including a lower pro-inflammatory status (less 'inflammaging' and autoimmunity) and a preserved humoral immune response (increased B cell-mediated immune response). Compared with controls, healthy centenarians also presented with a higher expression of proteins involved in angiogenesis and related to enhanced intercellular junctions, as well as a lower expression of proteins involved in cardiovascular abnormalities. The identification of these proteins/pathways might provide new insights into the biological mechanisms underlying the paradigm of healthy aging.engVoRhttp://creativecommons.org/licenses/by/4.0/ElderlyHealthy agingImmune systemProteomicsSenescenceAtribución 4.0 Internacional321007231243502-351510.18632/aging.1028261945-4589Agingopen access