Gambera, StefanoAbarrategi, AnderGarcia-Castro, JavierMorales-Molina, AlvaroRoma, JosepAlfranca, ArantzazuGonzalez-Camacho, Fernando2020-03-172020-03-172018Nat Commun. 2018 Sep 28;9(1):3994.2041-1723http://hdl.handle.net/20.500.12105/9262Factor de impacto: 14,919Osteosarcoma is a type of bone tumour characterized by considerable levels of phenotypic heterogeneity, aneuploidy, and a high mutational rate. The life expectancy of osteosarcoma patients has not changed during the last three decades and thus much remains to be learned about the disease biology. Here, we employ a RGB-based single-cell tracking system to study the clonal dynamics occurring in a de novo-induced murine osteosarcoma model. We show that osteosarcoma cells present initial polyclonal dynamics, followed by clonal dominance associated with adaptation to the microenvironment. Interestingly, the dominant clones are composed of subclones with a similar tumour generation potential when they are re-implanted in mice. Moreover, individual spontaneous metastases are clonal or oligoclonal, but they have a different cellular origin than the dominant clones present in primary tumours. In summary, we present evidence that osteosarcomagenesis can follow a neutral evolution model, in which different cancer clones coexist and propagate simultaneously.engVoRAnimalsBone NeoplasmsClone CellsLuminescent ProteinsMesenchymal Stem CellsMice, Inbred NODMice, KnockoutMice, SCIDMice, TransgenicMicroscopy, ConfocalOsteosarcomaSingle-Cell AnalysisAtribución 4.0 Internacional3026693391399410.1038/s41467-018-06401-z2041-1723Nature communicationsopen access