Carelli, ValerioHirano, MichioEnriquez, Jose AntonioChinnery, Patrick F2022-07-072022-07-072021-11-02Nat Rev Genet. 2021.http://hdl.handle.net/20.500.12105/14686Individual cells in the same induced pluripotent stem cell (iPSC)-derived clones can exhibit large heterogeneity. In this Comment, Carelli et al. discuss emerging evidence implicating variants in mitochondrial DNA, and highlight the need for routine screening of iPSCs. Single-cell analyses in recent years have shown major differences in the transcriptome between individual cells in the same induced pluripotent stem cell-derived clones. Although these differences are in part attributable to genetic and epigenetic modifications of the nuclear genome, emerging evidence suggests that variants in mitochondrial DNA also play a pivotal role.engVoRhttp://creativecommons.org/licenses/by-nc-nd/4.0/iPSMtDNAAttribution-NonCommercial-NoDerivatives 4.0 Internacional34728812 23269-7010.1038/s41576-021-00430-z1471-0064Nature reviews. Geneticsopen access