Puig-Serra, PilarCasado-Rosas, Maria CruzMartinez-Lage, MartaOlalla-Sastre, BeatrizAlonso-Yanez, AlejandroTorres-Ruiz, RaulRodriguez Perales, Sandra2025-01-282025-01-282022-02-03Int J Mol Sci . 2022 Feb 3;23(3):1757.https://hdl.handle.net/20.500.12105/26158This research was funded by the Spanish National Research and Development Plan, Instituto de Salud Carlos III, and FEDER (PI17/02303 and PI20/01837 to S.R.-P.; and PI21/01641 to R.T.-R.), Proyecto Desarrollo Tecnologico (DTS19/00111 to S.R.-P.), AEI/MICIU EXPLORA Project (BIO2017-91272-EXP to S.R.-P.), CaixaImpulse (CI18-00017; FuGe to S.R.-P.), and Asociacion Espanola contra el Cancer (AECC Lab 2020, LABAE20049RODR to S.R.-P.). The funders had no role in the study design, data collection and analysis, decision to publich nor preparation of the manuscript.CRISPR/Cas is a prokaryotic self-defense system, widely known for its use as a gene-editing tool. Because of their high specificity to detect DNA and RNA sequences, different CRISPR systems have been adapted for nucleic acid detection. CRISPR detection technologies differ highly among them, since they are based on four of the six major subtypes of CRISPR systems. In just 5 years, the CRISPR diagnostic field has rapidly expanded, growing from a set of specific molecular biology discoveries to multiple FDA-authorized COVID-19 tests and the establishment of several companies. CRISPR-based detection methods are coupled with pre-existing preamplification and readout technologies, achieving sensitivity and reproducibility comparable to the current gold standard nucleic acid detection methods. Moreover, they are very versatile, can be easily implemented to detect emerging pathogens and new clinically relevant mutations, and offer multiplexing capability. The advantages of the CRISPR-based diagnostic approaches are a short sample-to-answer time and no requirement of laboratory settings; they are also much more affordable than current nucleic acid detection procedures. In this review, we summarize the applications and development trends of the CRISPR/Cas13 system in the identification of particular pathogens and mutations and discuss the challenges and future prospects of CRISPR-based diagnostic platforms in biomedicine.engVoRhttp://creativecommons.org/licenses/by-nc-nd/4.0/CRISPRCas13diagnostichuman diseasesAttribution-NonCommercial-NoDerivatives 4.0 International351636782331757open access