Sá da Bandeira, DianaKilpatrick, Alastair MorrisMarques, MadalenaGomez-Salazar, MarioVentura, TelmaGonzalez, Zaniah NashiraStefancova, DorotaRossi, FionaVermeren, MatthieuVink, Chris SebastiaanBeltran, MarianaHenderson, Neil CowanJung, Bongnamvan der Linden, Reiniervan de Werken, Harmen Jan Georgevan Ijcken, Wilfred F JBetsholtz, ChristerForbes, Stuart JohnCuervo, HenarCrisan, Mihaela2024-01-172024-01-172022-07-19Cell Rep. 2022 Jul 19;40(3):111114.http://hdl.handle.net/20.500.12105/17202Hematopoietic stem cell (HSC) generation in the aorta-gonad-mesonephros region requires HSC specification signals from the surrounding microenvironment. In zebrafish, PDGF-B/PDGFRβ signaling controls hematopoietic stem/progenitor cell (HSPC) generation and is required in the HSC specification niche. Little is known about murine HSPC specification in vivo and whether PDGF-B/PDGFRβ is involved. Here, we show that PDGFRβ is expressed in distinct perivascular stromal cell layers surrounding the mid-gestation dorsal aorta, and its deletion impairs hematopoiesis. We demonstrate that PDGFRβ+ cells play a dual role in murine hematopoiesis. They act in the aortic niche to support HSPCs, and in addition, PDGFRβ+ embryonic precursors give rise to a subset of HSPCs that persist into adulthood. These findings provide crucial information for the controlled production of HSPCs in vitro.engVoRhttp://creativecommons.org/licenses/by-nc-nd/4.0/MesonephrosZebrafishAnimalsHematopoiesisHematopoietic Stem CellsMiceReceptor, Platelet-Derived Growth Factor betaStromal CellsAttribution-NonCommercial-NoDerivatives 4.0 Internacional3585855740311111410.1016/j.celrep.2022.1111142211-1247Cell reportsopen access