Oliva-Martinez, Jose LuisZarich-Dimitrievich, NatashaMartinez, NataliaJorge, RocíoCastrillo, AntonioAzañedo, MartaGarcia-Vargas, SusanaGutiérrez-Eisman, SilviaJuarranz, AngelesBoscá, LisardoGutkind, J SilvioRojas-Cabañeros, Jose Maria2020-04-232020-04-232004-08-06J Biol Chem. 2004 Aug 6;279(32):33480-91. Epub 2004 Jun 4.0021-9258http://hdl.handle.net/20.500.12105/9698Retraction in: The P34G mutation reduces the transforming activity of K-Ras and N-Ras in NIH 3T3 cells but not of H-Ras. [J Biol Chem. 2018]Ras proteins (H-, N-, and K-Ras) operate as molecular switches in signal transduction cascades controlling cell proliferation, differentiation, or apoptosis. The interaction of Ras with its effectors is mediated by the effector-binding loop, but different data about Ras location to plasma membrane subdomains and new roles for some docking/scaffold proteins point to signaling specificities of the different Ras proteins. To investigate the molecular mechanisms for these specificities, we compared an effector loop mutation (P34G) of three Ras isoforms (H-, N-, and K-Ras4B) for their biological and biochemical properties. Although this mutation diminished the capacity of Ras proteins to activate the Raf/ERK and the phosphatidylinositol 3-kinase/AKT pathways, the H-Ras V12G34 mutant retained the ability to cause morphological transformation of NIH 3T3 fibroblasts, whereas both the N-Ras V12G34 and the K-Ras4B V12G34 mutants were defective in this biological activity. On the other hand, although both the N-Ras V12G34 and the K-Ras4B V12G34 mutants failed to promote activation of the Ral-GDS/Ral A/PLD and the Ras/Rac pathways, the H-Ras V12G34 mutant retained the ability to activate these signaling pathways. Interestingly, the P34G mutation reduced specifically the N-Ras and K-Ras4B in vitro binding affinity to Ral-GDS, but not in the case of H-Ras. Thus, independently of Ras location to membrane subdomains, there are marked differences among Ras proteins in the sensitivity to an identical mutation (P34G) affecting the highly conserved effector-binding loop.engVoRhttp://creativecommons.org/licenses/by-nc-sa/4.0/AnimalsBinding SitesDNA-Binding ProteinsEnzyme ActivationGene ExpressionGenes, rasHumansMiceMitogen-Activated Protein Kinase 1Mitogen-Activated Protein Kinase 3Mitogen-Activated Protein KinasesNIH 3T3 CellsPhosphatidylinositol 3-KinasesPhospholipase DProtein IsoformsProtein-Serine-Threonine KinasesProto-Oncogene ProteinsProto-Oncogene Proteins c-aktProto-Oncogene Proteins c-rafSignal TransductionStructure-Activity RelationshipTranscription FactorsTransfectionets-Domain Protein Elk-1rac1 GTP-Binding Proteinral GTP-Binding Proteinsral Guanine Nucleotide Exchange Factorras ProteinsMutationAtribución-NoComercial-CompartirIgual 4.0 Internacional151810152793233480-9110.1074/jbc.M404058200The Journal of biological chemistryopen access