Ortega-García, María VictoriaSalguero, Francisco JavierGarcía, NereaDominguez-Rodriguez, MercedesMoreno-Iruela, InmaculadaBerrocal, Alexis2022-04-222022-04-222021-08-02Vet Med Sci. 2021; Aug. 1-6http://hdl.handle.net/20.500.12105/14159Background: Cutaneous forms of leishmaniosis due to Leishmania braziliensis have been reported in horses in the New World. Domestic animals play a role in the transmission of the disease. In Costa Rica, human cases of L. braziliensis, L. panamensis and L. infantum have been reported. Objectives: The present report describes five cases of equine cutaneous leishmaniosis in Costa Rica. The aetiological diagnosis was based on the presence of the parasite within the lesions. Methods: Skin biopsies were used to perform histopathological analyses of the lesions. Immunohistochemistry was used to detect the presence of the Leishmania spp. antigens in tissue sections. Laser-capture micro-dissection and quantitative real-time PCR techniques were carried out to detect the pathogen nucleic acid within the microscopic lesions. Results: Histopathological analyses showed a granulomatous inflammation within the dermis, with multi-nucleated giant cells, macrophages, lymphocytes and few neutrophils and eosinophils. We detected the parasite by immunohistochemistry, using a rabbit polyclonal antibody raised against Leishmania spp. However, we could not identify Leishmania spp. by quantitative real-time PCR in formalin-fixed paraffin-embedded tissues, using specific primers for the conserved region in the minicircle of the Leishmania DNA kinetoplast. Conclusions: Our results emphasise the importance of Leishmania spp. not only as a causative agent of equine cutaneous disease in the New World, but also as a possible emerging pathogen. Leishmaniosis is one of the most prevalent parasitic public health problems worldwide, and equines may have a role in the epidemiology of the disease.engVoRhttp://creativecommons.org/licenses/by-nc-nd/4.0/Leishmania sppCutaneous leishmaniosisHistopathologyHorseImmunohistochemistryLeishmaniosisAttribution-NonCommercial-NoDerivatives 4.0 Internacional34342158762234-223910.1002/vms3.5872053-1095Veterinary Medicine and Scienceopen access