Gille, AndreaStojnic, BojanDerwenskus, FelixTrautmann, AndreasSchmid-Staiger, UlrikePosten, ClemensBriviba, KarlisPalou, AndreuBonet, M LuisaRibot, Joan2024-09-102024-09-102019-04Gille A, Stojnic B, Derwenskus F, Trautmann A, Schmid-Staiger U, Posten C, et al. A Lipophilic Fucoxanthin-Rich Phaeodactylum tricornutum Extract Ameliorates Effects of Diet-Induced Obesity in C57BL/6J Mice. Nutrients. 2019 Apr;11(4):796.2072-6643http://hdl.handle.net/20.500.13003/17549https://hdl.handle.net/20.500.12105/22738Phaeodactylum tricornutum (P. tricornutum) comprise several lipophilic constituents with proposed anti-obesity and anti-diabetic properties. We investigated the effect of an ethanolic P. tricornutum extract (PTE) on energy metabolism in obesity-prone mice fed a high fat diet (HFD). Six- to eight-week-old male C57BL/6J mice were switched to HFD and, at the same time, received orally placebo or PTE (100 mg or 300 mg/kg body weight/day). Body weight, body composition, and food intake were monitored. After 26 days, blood and tissue samples were collected for biochemical, morphological, and gene expression analyses. PTE-supplemented mice accumulated fucoxanthin metabolites in adipose tissues and attained lower body weight gain, body fat content, weight of white adipose tissue (WAT) depots, and inguinal WAT adipocyte size than controls, independent of decreased food intake. PTE supplementation was associated with lower expression of Mest (a marker of fat tissue expandability) in WAT depots, lower gene expression related to lipid uptake and turnover in visceral WAT, increased expression of genes key to fatty acid oxidation and thermogenesis (Cpt1, Ucp1) in subcutaneous WAT, and signs of thermogenic activation including enhanced UCP1 protein in interscapular brown adipose tissue. In conclusion, these data show the potential of PTE to ameliorate HFD-induced obesity in vivo.enghttp://creativecommons.org/licenses/by/4.0/Phaeodactylum tricornutumMicroalgaeFucoxanthinEicosapentanoic acidObesityBrowningBrown adipose tissueAnimal FeedBlood GlucoseInsulin3T3-L1 CellsGene Expression RegulationMicroalgaeXanthophyllsMice, Inbred C57BLInsulin ResistanceObesityAdipose Tissue, BrownDietary FatsFatty Acids, NonesterifiedAnimalsLipid MetabolismDiet, High-FatDietary SupplementsMiceresearch articleAttribution 4.0 International3095993311479610.3390/nu11040796Nutrientsopen accessAnimalesMicroalgasCélulas 3T3-L1Resistencia a la InsulinaGrasas de la DietaÁcidos Grasos no EsterificadosXantófilasRegulación de la Expresión GénicaInsulinaTejido Adiposo PardoObesidadGlucemiaRatones Endogémicos C57BLAlimentación AnimalMetabolismo de los LípidosRatonesDieta Alta en GrasaSuplementos Dietéticos2-s2.0-85064537896467749800089L2001890909