Salgüero Fernandez, SergioBrochado-Kith, OscarVirseda-Berdices, AnaBerenguer, JuanGonzález-García, JuanMartinez, IsidoroDíez, CristinaHontañón, VíctorPérez-Latorre, LeireFernandez-Rodriguez, AmandaJimenez-Sousa, Maria AngelesResino, Salvador2023-05-172023-05-172023-03Biomed Pharmacother. 2023 Mar;159:114220.http://hdl.handle.net/20.500.12105/16074Corrigendum to "PBMCs gene expression signature of advanced cirrhosis with high risk for clinically significant portal hypertension in HIV/HCV coinfected patients: A cross-control study" [Biomed. Pharmacother. 159 (2023) 114220]. Biomed Pharmacother. 2023 Apr 27;114803. doi: 10.1016/j.biopha.2023.114803. PMID: 37120412.Background: Patients with advanced cirrhosis are at high risk of developing clinically significant portal hypertension (CSPH). We analyzed the gene expression profile of peripheral blood mononuclear cells (PBMCs) from HIV/HCV coinfected patients to identify a gene expression signature of advanced cirrhosis with high risk for CSPH. Methods: We conducted a cross-sectional study on 68 patients. Liver stiffness measurement (LSM) was used to stratify patients into < 12.5 kPa (no cirrhosis, n = 19), 12.5 - 24.9 kPa (cirrhosis, n = 20), and ≥ 25 kPa (advanced cirrhosis with high risk for CSPH, n = 29). Besides, we further evaluated LSM < 25 kPa (n = 39) vs. ≥ 25 kPa (n = 29). Total RNA was extracted from PBMCs, and poly(A) RNA sequencing was performed. Two significant differentially expressed (SDE) transcripts were validated by quantitative PCR in a different cohort (n = 46). Results: We found 60 SDE transcripts between patients with LSM < 12.5 kPa and ≥ 25 kPa. Partial least squares discriminant analysis showed that those 60 SDE transcripts collectively discriminated LSM ≥ 25 kPa, with an area under the receiver operating characteristic curve (AUROC) of 0.84. Eight genes had an AUROC ≥ 0.75 for LSM ≥ 25 kPa: five were positively associated with LSM values (SCAMP1, ABHD17B, GPR146, GTF2A1, and TMEM64), while three were inversely associated (ZFHX2-AS1, MDK, and STAG3L2). We validated the two SDE transcripts with the highest discrimination capacity in a different cohort, finding significant differences between < 25 kPa and ≥ 25 kPa (MDK (p = 0.006) and STAG3L2 (p = 0.021)). Conclusions: A gene expression signature of 60 transcripts was associated with advanced cirrhosis with high risk for CSPH in HIV/HCV coinfected patients.engVoRhttp://creativecommons.org/licenses/by/4.0/Advanced cirrhosisGene expressionHIV/HCV coinfectionLiver stiffnessPBMCsRNA-seqCoinfectionHIV InfectionsHypertension, PortalHepatitis CElasticity Imaging TechniquesHumansTranscriptomeCross-Sectional StudiesLeukocytes, MononuclearLiver CirrhosisLiverVesicular Transport ProteinsAtribución 4.0 Internacional3662881815911422010.1016/j.biopha.2023.1142201950-6007Biomedicine & pharmacotherapy = Biomedecine & pharmacotherapieopen access