Villanueva, Andrea ASánchez-Gómez, PilarMuñoz-Palma, ErnestoPuvogel, SofíaCasas, Bárbara SArriagada, CeciliaPeña-Villalobos, IsaacLois, PabloRamírez Orellana, ManuelLubieniecki, FabianaCasco Claro, FernandoGallegos, IvánGarcia-Castro, JavierTorres, Vicente APalma, Verónica2021-04-152021-04-152021Cell Adh Migr. 2021 Dec;15(1):58-73.http://hdl.handle.net/20.500.12105/12663Neuroblastoma is a highly metastatic tumor that emerges from neural crest cell progenitors. Focal Adhesion Kinase (FAK) is a regulator of cell migration that binds to the receptor Neogenin-1 and is upregulated in neuroblastoma. Here, we show that Netrin-1 ligand binding to Neogenin-1 leads to FAK autophosphorylation and integrin β1 activation in a FAK dependent manner, thus promoting neuroblastoma cell migration. Moreover, Neogenin-1, which was detected in all tumor stages and was required for neuroblastoma cell migration, was found in a complex with integrin β1, FAK, and Netrin-1. Importantly, Neogenin-1 promoted neuroblastoma metastases in an immunodeficient mouse model. Taken together, these data show that Neogenin-1 is a metastasis-promoting protein that associates with FAK, activates integrin β1 and promotes neuroblastoma cell migration.engVoRhttp://creativecommons.org/licenses/by/4.0/FAKCell migrationNeogenin-1Netrin-1Integrin-β1 activatioMetastasisNeuroblastomaAtribución 4.0 Internacional3372415015158-7310.1080/19336918.2021.18923971933-6926Cell adhesion & migrationopen access