Pons, Daniel-GabrielMoran, CarmenAlorda-Clara, MarinaOliver, JordiRoca, PilarSastre-Serra, Jorge2024-09-132024-09-132020-03-24Pons DG, Moran C, Alorda-Clara M, Oliver J, Roca P, Sastre-Serra J. Micronutrients Selenomethionine and Selenocysteine Modulate the Redox Status of MCF-7 Breast Cancer Cells. Nutrients. 2020 Mar 24;12(3).http://hdl.handle.net/20.500.13003/18256https://hdl.handle.net/20.500.12105/22953Selenium is a micronutrient which is found in many foods, with redox status modulation activity. Our aim was to evaluate the effects of two chemical forms of selenoamino acids, Seleno-L-methionine and Seleno-L-cystine (a diselenide derived from selenocysteine), at different concentrations on cell viability, hydrogen peroxide production, antioxidant enzymes, UCP2 protein expression, as well as lipid and protein oxidative damage in MCF-7 breast cancer cells. Results showed that Seleno-L-methionine did not cause an increase in hydrogen peroxide production at relatively low concentrations, accompanied by a rise in the antioxidant enzymes catalase and MnSOD, and UCP2 protein expression levels. Furthermore, a decrease in protein and lipid oxidative damage was observed at 10 µM concentration. Otherwise, Seleno-L-cystine increased hydrogen peroxide production from relatively low concentrations (100 nM) to a large increase at high concentrations. Moreover, at 10 µM, Seleno-L-cystine decreased UCP2 and MnSOD protein expression. In conclusion, the chemical form of selenoamino acid and their incorporation to selenoproteins could affect the regulation of the breast cancer cell redox status. Taken together, the results obtained in this study imply that it is important to control the type of selenium-enriched nutrient consumption, taking into consideration their composition and concentration.enghttp://creativecommons.org/licenses/by/4.0/AntioxidantsBreast NeoplasmsOxidative StressReactive Oxygen SpeciesHumansHydrogen PeroxideMCF-7 CellsSelenomethionineCell SurvivalFemaleUncoupling Protein 2Oxidation-ReductionMicronutrientsLipid MetabolismSelenocysteineresearch articleAttribution 4.0 International3221388312310.3390/nu120308652072-6643Nutrientsopen accessSelenocisteínaMicronutrientesFemeninoCélulas MCF-7Supervivencia CelularPeróxido de HidrógenoHumanosSelenometioninaEspecies Reactivas de OxígenoProteína Desacopladora 2Estrés OxidativoAntioxidantesNeoplasias de la MamaMetabolismo de los LípidosOxidación-Reducción2-s2.0-85082487345531831000274L2004100643