Torrente-Rodríguez, Rebeca MMontero-Calle, Ana MariaSan Bartolomé, ClaraCano, OlgaVazquez-Alcaraz, MonicaIglesias-Caballero, MariaCorral-Lugo, AndresMcConnell, Michael JPascal, MarionaMas-Lloret, VicentePingarrón, José MBarderas Manchado, RodrigoCampuzano, Susana2022-11-152022-11-152022-07-11Angew Chem Int Ed Engl. 2022 Jul 11;61(28):e202203662.http://hdl.handle.net/20.500.12105/15150The development of versatile and sensitive biotools to quantify specific SARS-CoV-2 immunoglobulins in SARS-CoV-2 infected and non-infected individuals, built on the surface of magnetic microbeads functionalized with nucleocapsid (N) and in-house expressed recombinant spike (S) proteins is reported. Amperometric interrogation of captured N- and S-specific circulating total or individual immunoglobulin (Ig) isotypes (IgG, IgM, and IgA), subsequently labelled with HRP-conjugated secondary antibodies, was performed at disposable single or multiplexed (8×) screen-printed electrodes using the HQ/HRP/H2 O2 system. The obtained results using N and in-house expressed S ectodomains of five SARS-CoV-2 variants of concern (including the latest Delta and Omicron) allow identification of vulnerable populations from those with natural or acquired immunity, monitoring of infection, evaluation of vaccine efficiency, and even identification of the variant responsible for the infection.engVoRhttps://creativecommons.org/licenses/by-nc/4.0/Electroanalytical BioplatformsImmune ResponseOmicronSARS-CoV-2Viral AntigensBiosensing TechniquesCOVID-19Antibodies, ViralCOVID-19 TestingHumansImmunityImmunoglobulin GSARS-CoV-2Spike Glycoprotein, CoronavirusAttribution-NonCommercial 4.0 Internacional355075736128e20220366210.1002/anie.2022036621521-3773Angewandte Chemie (International ed. in English)open access