de Lucas, BeatrizBernal, AuroraPerez, Laura M.San Martin, NuriaGalvez, Beatriz G.2017-10-302017-10-302016PLoS One. 2016; 11(3):e01500041932-6203http://hdl.handle.net/20.500.12105/5242Mesenchymal precursors (MPs) present some advantageous features, such as differentiation and migration, which make them promising candidates for cell therapy. A better understanding of MP migration characteristics would aid the development of cell delivery protocols. Traditionally, cell migration is thought to occur only through the formation of lamellipodia. More recently, contractility-driven bleb formation has emerged as an alternative mechanism of motility. Here we report that MPs derived from different tissues present spontaneously dynamic cytoplasmic projections in sub-confluent culture, which appear as a combination of lamellipodia with blebs in the leading edge. Upon initial seeding, however, only bleb structures could be observed. Immunofluorescence revealed the presence of pERM, RhoA and F-actin during the blebbing process. Results from migration assays in the presence of blebbistatin, a myosin II inhibitor, showed that bleb formation correlated with migratory capacity, suggesting a functional role for blebs in migration. Bleb formation might be a useful mechanism to improve cell migration in cellular therapy protocols.engVoRhttp://creativecommons.org/licenses/by/4.0/STEM-CELLSCARDIAC MESOANGIOBLASTSAMEBOID MIGRATIONSKELETAL-MUSCLEINVASIONBLEBSCONTRACTILITYLAMELLIPODIABIOMECHANICSMECHANICSAtribución 4.0 Internacional269304661110.1371/journal.pone.0150004PLoS Oneopen access