Torres-Ruiz, RaulGrazioso, Tatiana PBrandt, MartaMartinez-Lage, MartaRodriguez Perales, SandraDjouder, Nabil2024-02-082024-02-082021-09-17STAR Protoc . 2021;2(3):100631.http://hdl.handle.net/20.500.12105/17562Chromosomal instability (CIN), a type of genomic instability, favors changes in chromosome number and structure and it is associated with the progression and initiation of multiple diseases, including cancer. Therefore, CIN identification and analysis represents a useful tool for cancer diagnosis and treatment. Here, we report an optimized molecular cytogenetic protocol to detect CIN in formalin-fixed, paraffin-embedded mouse and human tissues, using fluorescent in situ hybridization to visualize and quantify chromosomal alterations such as amplifications, deletions, and translocations. For complete information on the generation and use of this protocol, please refer to Brandt et al. (2018).engVoRhttp://creativecommons.org/licenses/by-nc-nd/4.0/Chromosomal InstabilityInterphaseAnimalsChromosomes, Artificial, BacterialDNAElectrophoresis, Agar GelIn Situ Hybridization, FluorescenceMiceParaffin EmbeddingAttribution-NonCommercial-NoDerivatives 4.0 Internacional342585922310063110.1016/j.xpro.2021.1006312666-1667STAR protocolsopen access