Alfranca, ArantzazuCampanero, Miguel RRedondo, Juan Miguel2020-04-292020-04-292018-10Trends Mol Med. 2018; 24(10):852-3714714914http://hdl.handle.net/20.500.12105/9808Lentiviral vectors (LVs) transduce quiescent cells and provide stable integration to maintain transgene expression. Several approaches have been adopted to optimize LV safety profiles. Similarly, LV targeting has been tailored through strategies including the modification of envelope components, the use of specific regulatory elements, and the selection of appropriate administration routes. Models of aortic disease based on a single injection of pleiotropic LVs have been developed that efficiently transduce the three aorta layers in wild type mice. This approach allows the dissection of pathways involved in aortic aneurysm formation and the identification of targets for gene therapy in aortic diseases. LVs provide a fast, efficient, and affordable alternative to genetically modified mice to study disease mechanisms and develop therapeutic tools.engAMhttp://creativecommons.org/licenses/by-nc-nd/4.0/Aortic aneurysmDisease modelsGene therapyLentiviral vectorsViral targetingADAMTS1 ProteinAnimalsAortic AneurysmClinical Trials as TopicDisease Models, AnimalGene Expression RegulationGenetic TherapyGenetic VectorsHumansImmunity, InnateLentivirusMicePatient SafetyRNA, Small InterferingTransduction, GeneticTransgenesAttribution-NonCommercial-NoDerivatives 4.0 Internacional302137012410825-83710.1016/j.molmed.2018.08.0011471-499XTrends in molecular medicineopen access