Xufré, CristinaGonzález, TaníaLeal, LornaTrubey, Charles MLifson, Jeffrey DGatell, José MaríaAlcamí, JoséCliment, NúriaGarcía, FelipeSánchez-Palomino, Sonsoles2022-12-202022-12-202022-04Front Virol. 2022 Apr;2:869431.http://hdl.handle.net/20.500.12105/15287We described a novel HIV autologous isolation method based in coculturing macrophages and CD4+T-cell-enriched fractions from peripheral blood collected from antiretroviral-treated (ART) HIV patients. This method allows the isolation of high viral titers of autologous viruses, over 1010HIV RNA copies/ml, and reduces the time required to produce necessary amounts for virus for use as antigens presented by monocyte-derived myeloid cells in HIV therapeutic vaccine approaches. By applying these high titer and autologous virus produced in the patient-derived cells, we intended to elicit a boost of the immunological system response in HIV therapeutic vaccines in clinical trials.engVoRhttp://creativecommons.org/licenses/by/4.0/CD4+ T cellsHIV isolationHIV therapeutic vaccineAutologous HIV immunogenCocultureMonocyte-derived macrophages (MDM)Atribución 4.0 Internacional35967461286943110.3389/fviro.2022.8694312673-818XFrontiers in Virologyopen access