Torralba, DanielBaixauli, FrancescVillarroya-Beltri, CarolinaFernandez-Delgado, IreneLatorre-Pellicer, AnaAcin-Perez, RebecaMartin-Cofreces, Noa B.Jaso-Tamame, Angel LuisIborra, SalvadorJorge, InmaculadaGonzalez-Aseguinolaza, GloriaGaraude, JohanVicente-Manzanares, MiguelEnriquez, Jose AntonioMittelbrunn, MariaSanchez-Madrid, Francisco2018-11-052018-11-052018Nat Commun. 2018; 9(1):26582041-1723http://hdl.handle.net/20.500.12105/6572Interaction of T cell with antigen-bearing dendritic cells (DC) results in T cell activation, but whether this interaction has physiological consequences on DC function is largely unexplored. Here we show that when antigen-bearing DCs contact T cells, DCs initiate antipathogenic programs. Signals of this interaction are transmitted from the T cell to the DC, through extracellular vesicles (EV) that contain genomic and mitochondrial DNA, to induce antiviral responses via the cGAS/STING cytosolic DNA-sensing pathway and expression of IRF3-dependent interferon regulated genes. Moreover, EV-treated DCs are more resistant to subsequent viral infections. In summary, our results show that T cells prime DCs through the transfer of exosomal DNA, supporting a specific role for antigen-dependent contacts in conferring protection to DCs against pathogen infection. The reciprocal communication between innate and adaptive immune cells thus allow efficacious responses to unknown threats.engVoRhttp://creativecommons.org/licenses/by/4.0/MITOCHONDRIAL-DNAIMMUNOLOGICAL SYNAPSEIMMUNE-RESPONSESCANCER-CELLSIN-VIVOKAPPA-BEXOSOMESRELEASEINNATEIDENTIFICATIONAtribución 4.0 Internacional29985392910.1038/s41467-018-05077-9Nature Communicationsopen access