Gonzalez Portal, Maria EugeniaCarrasco, Luis2019-06-202019-06-202005González M.E., Carrasco L. (2005) Viral Proteins that Enhance Membrane Permeability. In: Fischer W.B. (eds) Viral Membrane Proteins: Structure, Function, and Drug Design. Protein Reviews, vol 1. Springer, Boston, MA978-0-387-28146-9978-0-306-48495-7978-0-306-48495-7http://hdl.handle.net/20.500.12105/7792This chapter is devoted to reviewing some characteristics of membrane permeabilization by viral proteins. In addition, the methodology used to assay enhanced permeability in animal cells is described. Finally, the design of selective viral inhibitors based on the modification of cellular membranes during virus entry or at late times of infection is also discussed.1. Introduction 2. Measuring alterations in membrane permeability 2.1. The hygromycin B test 2.2. Entry of macromolecules into virus-infected cells 2.3. Other assays to test the entry or exit of macromolecules from virus-infected cells 3. Viral proteins that modify permeability 3.1. Viroporins 3.2. Viral glycoproteins that modify membrane permeability 4. Membrane permeabilization and drug design 4.1. Antibiotics and toxins that selectively enter virus-infected cells 4.2. Viroporin inhibitors 4.3. Antiviral agents that interfere with viral glycoproteins 5. Acknowledgments References FiguresengSMURhttp://creativecommons.org/licenses/by-nc-sa/4.0/ViroporinsViral GlycoproteinsMembrane permeabilizationAntiviral agentsViroporin inhibitorsBiological assaysHIV-1PoliovirusHIV-1 gp41Virus ReleaseCell Membrane PermeabilityCytopathogenic Effect, ViralModels, BiologicalMembrane ProteinsMembrane GlycoproteinsToxins, BiologicalAtribución-NoComercial-CompartirIgual 4.0 Internacional19010.1007/0-387-28146-0_6Protein Reviews, Vol Iopen access