Culemann, StephanKnab, KatharinaEuler, MaximilienWegner, AnjaGaribagaoglu, HilalAckermann, JochenFischer, KimKienhöfer, DeborahCrainiciuc, GeorgianaHahn, JonasGrüneboom, AnikaNimmerjahn, FalkUderhardt, StefanHidalgo, AndresSchett, GeorgHoffmann, Markus HKrönke, Gerhard2024-05-082024-05-082023-06-05J Exp Med. 2023 Jun 5;220(6):e20220525.http://hdl.handle.net/20.500.12105/19290Clodronate liposomes (Clo-Lip) have been widely used to deplete mononuclear phagocytes (MoPh) to study the function of these cells in vivo. Here, we revisited the effects of Clo-Lip together with genetic models of MoPh deficiency, revealing that Clo-Lip exert their anti-inflammatory effects independent of MoPh. Notably, not only MoPh but also polymorphonuclear neutrophils (PMN) ingested Clo-Lip in vivo, which resulted in their functional arrest. Adoptive transfer of PMN, but not of MoPh, reversed the anti-inflammatory effects of Clo-Lip treatment, indicating that stunning of PMN rather than depletion of MoPh accounts for the anti-inflammatory effects of Clo-Lip in vivo. Our data highlight the need for a critical revision of the current literature on the role of MoPh in inflammation.engVoRhttp://creativecommons.org/licenses/by-sa/4.0/LiposomesClodronic AcidHumansNeutrophilsInflammationAnti-Inflammatory AgentsAtribución-CompartirIgual 4.0 Internacional36976180220610.1084/jem.202205251540-9538The Journal of experimental medicineopen access