Enamorado, MichelIborra, SalvadorPriego, ElenaCueto, Francisco J.Quintana, Juan A.Martinez-Cano, SaraiMejias-Perez, ErnestoEsteban, MarianoMelero, IgnacioHidalgo, AndresSancho, David2017-10-202017-10-202017Nat Commun. 2017; 8:160732041-1723http://hdl.handle.net/20.500.12105/5105The goal of successful anti-tumoural immunity is the development of long-term protective immunity to prevent relapse. Infiltration of tumours with CD8(+) T cells with a resident memory (Trm) phenotype correlates with improved survival. However, the interplay of circulating CD8(+) T cells and Trm cells remains poorly explored in tumour immunity. Using different vaccination strategies that fine-tune the generation of Trm cells or circulating memory T cells, here we show that, while both subsets are sufficient for anti-tumour immunity, the presence of Trm cells improves anti-tumour efficacy. Transferred central memory T cells (Tcm) generate Trm cells following viral infection or tumour challenge. Anti-PD-1 treatment promotes infiltration of transferred Tcm cells within tumours, improving anti-tumour immunity. Moreover, Batf3-dependent dendritic cells are essential for reactivation of circulating memory anti-tumour response. Our findings show the plasticity, collaboration and requirements for reactivation of memory CD8(+) T cells subsets needed for optimal tumour vaccination and immunotherapy.engVoRhttp://creativecommons.org/licenses/by/4.0/TUMOR-INFILTRATING LYMPHOCYTESCD8-ALPHA(+) DENDRITIC CELLSTISSUE-RESIDENTNONLYMPHOID TISSUEVIRAL-INFECTIONRESPONSESREVEALSCANCERIL-12EXPRESSIONAtribución 4.0 Internacional28714465810.1038/ncomms16073NATURE COMMUNICATIONSopen access