Costa, Andreavan der Stelt, IngeReynés, BárbaraKonieczna, JadwigaFiol Sala, MiquelKeijer, JaapPalou, AndreuRomaguera, Doravan Schothorst, Evert MOliver, Paula2024-10-092024-10-092023-02Costa A, van der Stelt I, Reynés B, Konieczna J, Fiol M, Keijer J, et al. Whole-Genome Transcriptomics of PBMC to Identify Biomarkers of Early Metabolic Risk in Apparently Healthy People with Overweight-Obesity and in Normal-Weight Subjects. Mol Nutr Food Res. 2022 Dec;e2200503.https://hdl.handle.net/20.500.13003/18827https://hdl.handle.net/20.500.12105/23805Scope: Peripheral blood mononuclear cells (PBMC) provide a useful and minimally invasive source of biomarkers. Here to identify PBMC transcriptomic biomarkers predictive of metabolic impairment related to increased adiposity is aimed. Methods and results: The study analyzed the global PBMC transcriptome in metabolically healthy (normoglycemic) volunteers with overweight-obesity (OW-OB, n = 12), and in subjects with metabolically obese normal-weight (MONW, n = 5) phenotype, in comparison to normal-weight (NW, n = 12) controls. The study identifies 1072 differentially expressed genes (DEGs) in OW-OB versus NW and 992 in MONW versus NW. Hierarchical clustering of the top 100 DEGs clearly distinguishes OW-OB and MONW from NW. Remarkably, the OW-OB and MONW phenotypes share 257 DEGs regulated in the same direction. The top up-regulated gene CXCL8, coding for interleukin 8, with a role in obesity-related pathologies, is of special interest as a potential marker for predicting increased metabolic risk. CXCL8 expression is increased mainly in the MONW group and correlated directly with C-reactive protein levels. Conclusions: PBMC gene expression analysis of CXCL8 or a pool of DEGs may be used to identify early metabolic risk in an apparently healthy population regardless of their BMI, i.e., subjects with OW-OB or MONW phenotype and to apply adequate and personalized nutritional preventive strategies.enghttp://creativecommons.org/licenses/by-nc-nd/4.0/TranscriptomeBiomarkersGene Expression ProfilingHumansBody Mass IndexOverweightLeukocytes, MononuclearObesityresearch articleAttribution-NonCommercial-NoDerivatives 4.0 Internacional36564895674e220050310.1002/mnfr.2022005031613-4133Molecular nutrition & food researchopen accessÍndice de Masa CorporalHumanosObesidadLeucocitos MononuclearesBiomarcadoresPerfilación de la Expresión GénicaSobrepesoTranscriptoma2-s2.0-85147267084921501800001L639858010