Negredo, AnabelHabela, Miguel ÁngelRamirez de Arellano, EvaDíez-Fuertes, FranciscoLasala, FátimaLópez, PabloLasala, FátimaSarriá, AnaLabiod, NuriaCalero-Bernal, RafaelArenas, MiguelTenorio, AntonioEstrada-Peña, AgustínSánchez-Seco, María Paz2025-01-102025-01-102019-06Negredo A, Habela MÁ, Ramírez de Arellano E, Diez F, Lasala F, López P, Sarriá A, Labiod N, Calero-Bernal R, Arenas M, Tenorio A, Estrada-Peña A, Sánchez-Seco MP. Survey of Crimean-Congo Hemorrhagic Fever Enzootic Focus, Spain, 2011-2015. Emerg Infect Dis. 2019 Jun;25(6):1177-1184.1080-6040https://hdl.handle.net/20.500.12105/25992During 2011-2015, we conducted a Crimean-Congo hemorrhagic fever virus (CCHFV) survey in captured ticks that were feeding mainly on wild and domestic ungulates in Spain, where presence of this virus had been reported previously. We detected CCHFV RNA in Hyalomma lusitanicum and H. marginatum ticks for 3 of the 5 years. The rate of infected ticks was 2.78% (44/1,579), which was similar to those for other countries in Europe with endemic foci for CCHFV (Kosovo, Bulgaria, and Albania). These data confirm the established spread of CCHFV into western Europe. Phylogenetic study of the small RNA segment showed Africa-3 clade as the only genotype identified, although we observed cocirculation of genetic variants during 2011 and 2015. We could not rule out genetic reassortments because of lack of sequence data for the medium and large RNA segments of the virus genome.engVoRhttp://creativecommons.org/licenses/by/4.0/CCHFVCrimean Congo hemorrhagic fever virusCrimean-Congo hemorrhagic feverHyalomma spp. ticksSpainEndemic circulationEnzootic focusMolecular epidemiology surveyPhylogenetic analysisReverse transcription PCRTick-borne infectionsVector-borne infectionsVirusesZoonosesAnimalsArthropod VectorsGenome, ViralGeographyHemorrhagic Fever Virus, Crimean-CongoHemorrhagic Fever, CrimeanHumansPhylogenyPublic Health SurveillanceSpainTicksZoonosesSurvey of Crimean-Congo Hemorrhagic Fever Enzootic Focus, Spain, 2011-2015Attribution 4.0 International311072192561177-118410.3201/eid2506.1808771080-6059Emerging infectious diseasesopen access