Sliepen, KwintenHan, Byung WooBontjer, IljaMooij, PetraGarces, FernandoBehrens, Anna-JaninaRantalainen, KimmoKumar, SonuSarkar, AnitaBrouwer, Philip J MHua, YuanziTolazzi, MonicaSchermer, EdithTorres, Jonathan LOzorowski, Gabrielvan der Woude, PatriciaTorrents de la Peña, Albavan Breemen, Mariëlle JCamacho-Sánchez, Juan MiguelBurger, Judith AMedina-Ramírez, MaxGonzalez-Fernandez, NuriaAlcamí, JoséLaBranche, CeliaScarlatti, Gabriellavan Gils, Marit JCrispin, MaxMontefiori, David CWard, Andrew BKoopman, GerritMoore, John PShattock, Robin JBogers, Willy MWilson, Ian ASanders, Rogier W2020-03-172020-03-172019Nat Commun. 2019 May 29;10(1):23552041-1723http://hdl.handle.net/20.500.12105/9255Stabilized HIV-1 envelope glycoproteins (Env) that resemble the native Env are utilized in vaccination strategies aimed at inducing broadly neutralizing antibodies (bNAbs). To limit the exposure of rare isolate-specific antigenic residues/determinants we generated a SOSIP trimer based on a consensus sequence of all HIV-1 group M isolates (ConM). The ConM trimer displays the epitopes of most known bNAbs and several germline bNAb precursors. The crystal structure of the ConM trimer at 3.9 Å resolution resembles that of the native Env trimer and its antigenic surface displays few rare residues. The ConM trimer elicits strong NAb responses against the autologous virus in rabbits and macaques that are significantly enhanced when it is presented on ferritin nanoparticles. The dominant NAb specificity is directed against an epitope at or close to the trimer apex. Immunogens based on consensus sequences might have utility in engineering vaccines against HIV-1 and other viruses.engVoRhttp://creativecommons.org/licenses/by/4.0/AIDS VaccinesAnimalsAntibodies, NeutralizingConsensus SequenceEpitopesHIV AntibodiesHIV-1MacacaProtein MultimerizationRabbitsenv Gene Products, Human Immunodeficiency VirusAtribución 4.0 Internacional31142746101235510.1038/s41467-019-10262-52041-1723Nature communicationsopen access