Rivaya, BelenLe Roy, ChloeJordana-Lluch, ElenaFernandez-Rivas, GemaCasan, CristinaGonzalez, VictoriaWang-Wang, Jun HaoBebear, CecileMatas, LurdesPereyre, Sabine2024-10-042024-10-042022-04Rivaya B, Le Roy C, Jordana-Lluch E, Fernandez-Rivas G, Casan C, Gonzalez V, et al. Detection and Prevalence of Macrolide and Fluoroquinolone Resistance in Mycoplasma genitalium in Badalona, Spain. Antibiotics-Basel. 2022 Apr;11(4):485.2079-6382https://hdl.handle.net/20.500.13003/19530https://hdl.handle.net/20.500.12105/23410Macrolide and fluoroquinolone resistance (MLr/FQr) in Mycoplasma genitalium (MG) infections is concerning worldwide. Current guidelines recommend performing MLr detection in MG-positive cases to adjust antimicrobial therapy. We aimed to evaluate the usefulness of PCR followed by pyrosequencing for MLr detection in comparison with a one-step commercial assay and to assess the prevalence of MLr and FQr in Badalona, Spain. A total of 415 MG-positive samples by Allplex STI-7 (Seegene) were analyzed for MLr detection by pyrosequencing. From those, 179 samples were further analyzed for MG and MLr by ResistancePlus® MG kit (SpeeDx) and 100 of them also for fluoroquinolone resistance (FQr) by sequencing the parC gene. Regarding MG detection, Allplex and Resistance Plus® showed an overall agreement of 87%, but this value rose to 95.4% if we compare them for MLr detection. Prevalence of MLr was 23.1% in Badalona, but this rate increased to 73.7% in the HIV-positive patients cohort. FQr detection showed 3% of resistant strains. Pyrosequencing is a convenient and cheap technique for MLr detection, but one-step tools should be considered in high-throughput laboratories. Despite the fact that MLr remained moderate and FQr was low in our study, simultaneous MG and MLr detection would improve patient's management applying resistance-guided treatment strategies.enghttp://creativecommons.org/licenses/by/4.0/Mycoplasma genitaliumMacrolideFluoroquinoloneResistanceSpainresearch articleAttribution 4.0 International3545323611448510.3390/antibiotics11040485Antibiotics-Baselopen access2-s2.0-85128472828785540700001L2016414761