Cuervo, HenarGuerrero, Néstor ACarbajosa, SofíaBeschin, AlainDe Baetselier, PatrickGironès, NúriaFresno, Manuel2024-01-172024-01-172011-09-01J Immunol. 2011 Sep 1;187(5):2656-65.http://hdl.handle.net/20.500.12105/17193Chagas disease, caused by the protozoan parasite Trypanosoma cruzi, affects several million people in Latin America. Myocarditis, observed in the acute and chronic phases of the disease, is characterized by a mononuclear cell inflammatory infiltrate. We previously identified a myeloid cell population in the inflammatory heart infiltrate of infected mice that expressed arginase I. In this study, we purified CD11b(+) myeloid cells from the heart and analyzed their phenotype and function. Those CD11b(+) cells were ∼70% Ly6G(-)Ly6C(+) and 25% Ly6G(+)Ly6C(+). Moreover, purified CD11b(+)Ly6G(-) cells, but not Ly6G(+) cells, showed a predominant monocytic phenotype, expressed arginase I and inducible NO synthase, and suppressed anti-CD3/anti-CD28 Ab-induced T cell proliferation in vitro by an NO-dependent mechanism, activity that best defines myeloid-derived suppressor cells (MDSCs). Contrarily, CD11b(+)Ly6G(+) cells, but not CD11b(+)Ly6G(-) cells, expressed S100A8 and S100A9, proteins known to promote recruitment and differentiation of MDSCs. Together, our results suggest that inducible NO synthase/arginase I-expressing CD11b(+)Ly6G(-) myeloid cells in the hearts of T. cruzi-infected mice are MDSCs. Finally, we found plasma l-arginine depletion in the acute phase of infection that was coincident in time with the appearance of MDSCs, suggesting that in vivo arginase I could be contributing to l-arginine depletion and systemic immunosuppression. Notably, l-arginine supplementation decreased heart tissue parasite load, suggesting that sustained arginase expression through the acute infection is detrimental for the host. This is, to our knowledge, the first time that MDSCs have been found in the heart in the context of myocarditis and also in infection by T. cruzi.engVoRAnimalsArginaseArginineCD11b AntigenCell SeparationChagas CardiomyopathyFlow CytometryMiceMice, Inbred BALB CMice, Inbred C57BLMyeloid CellsNitric Oxide Synthase Type IIRNA, MessengerReverse Transcriptase Polymerase Chain ReactionTrypanosoma cruziAttribution-NonCommercial-NoDerivatives 4.0 Internacional218040131875265610.4049/jimmunol.10029281550-6606Journal of immunology (Baltimore, Md. : 1950)open access