Delgado-Chaves, Fernando MMartínez-García, Pedro ManuelHerrero-Ruiz, AndrésGómez-Vela, FranciscoDivina, FedericoJimeno-González, SilviaCortes-Ledesma, Felipe2024-03-152024-03-152022-10Data Brief . 2022 Aug 1:44:108499.http://hdl.handle.net/20.500.12105/18960Type II DNA topoisomerases relax topological stress by transiently gating DNA passage in a controlled cut-and-reseal mechanism that affects both DNA strands. Therefore, they are essential to overcome topological problems associated with DNA metabolism. Their aberrant activity results in the generation of DNA double-strand breaks, which can seriously compromise cell survival and genome integrity. Here, we profile the transcriptome of human-telomerase-immortalized retinal pigment epithelial 1 (RPE-1) cells when treated with merbarone, a drug that catalytically inhibits type II DNA topoisomerases. We performed RNA-Seq after 4 and 8 h of merbarone treatment and compared transcriptional profiles versus untreated samples. We report raw sequencing data together with lists of gene counts and differentially expressed genes.engVoRhttp://creativecommons.org/licenses/by-nc-nd/4.0/Attribution-NonCommercial-NoDerivatives 4.0 Internacional359831304410849910.1016/j.dib.2022.1084992352-3409Data in briefopen access