Targeted gene disruption of the 14-alpha sterol demethylase (cyp51A) in Aspergillus fumigatus and its role in azole drug susceptibility

Mellado, EmiliaGarcia-Effron, GuillermoBuitrago, Maria JoseAlcazar-Fuoli, LauraCuenca-Estrella, ManuelRodriguez-Tudela, Juan Luis2019-05-312019-05-312005-06Antimicrob Agents Chemother. 2005;49(6):2536-8.0066-4804http://hdl.handle.net/20.500.12105/7702The role of Aspergillus fumigatus 14alpha-sterol demethylase (Cyp51A) in azole drug susceptibility was assessed. Targeted disruption of cyp51A in azole-susceptible and -resistant strains decreased MICs from 2- to 40-fold. The cyp51A mutants were morphologically indistinguishable from the wild-type strain, retaining the ability to cause pulmonary disease in neutropenic mice.engAMhttp://creativecommons.org/licenses/by-nc-sa/4.0/Targeted gene disruption of the 14-alpha sterol demethylase (cyp51A) in Aspergillus fumigatus and its role in azole drug susceptibilityAtribución-NoComercial-CompartirIgual 4.0 Internacional159175664962536-810.1128/AAC.49.6.2536-2538Antimicrobial agents and chemotherapyopen access