Cordoba, AlbaManzanaro-Moreno, NahuelColom, CarmeRonold, Hans JLyngstadaas, Staale PMonjo, MartaRamis, Joana Maria2024-09-062024-09-062018-11Cordoba A, Manzanaro-Moreno N, Colom C, Ronold Hans J, Lyngstadaas Staale P, Monjo M, et al. Quercitrin Nanocoated Implant Surfaces Reduce Osteoclast Activity In Vitro and In Vivo. Int J Mol Sci. 2018 Nov;19(11):3319.1422-0067http://hdl.handle.net/20.500.13003/9037https://hdl.handle.net/20.500.12105/22586In this study, the effect on osteoclast activity in vitro and in vivo of titanium implants that were coated with quercitrin was evaluated. Titanium surfaces were covalently coated with the flavonoid quercitrin. The effect of the surfaces on osteoclastogenesis was first tested in vitro on RAW264.7 cells that were supplemented with receptor activator of nuclear factor kappa-B ligand (RANKL) to generate osteoclast-like cells by tartrate-resistant acid phosphatase (TRAP) inmunostaining after five days of culture, and by analysis of the mRNA expression levels of markers related to bone resorption after seven days of culture. A rabbit tibial model was used to evaluate the in vivo biological response to the implant surfaces after eight weeks of healing, analyzing the lactate dehydrogenase (LDH) and the alkaline phosphatase (ALP) activities in the wound fluid that were present at the implant interface and the peri-implant bone mRNA expression levels of several markers related to inflammation, bone resorption and osteoblast-osteoclast interaction. No differences between groups and control surfaces were found in the wound fluid analyses. Moreover, quercitrin implant surfaces significantly decreased the expression of osteoclast related genes in vitro (Trap, CalcR, Ctsk, H(+)ATPase, Mmp9) and in vivo (Ctsk, H(+)ATPase, Mmp9) as well as the expression of RankL in vivo. Moreover, quercitrin surfaces were not cytotoxic for the cells. Thus, quercitrin implant surfaces were biocompatible and decreased osteoclastogenesis in vitro and in vivo. This could be used to improve the performance of dental implants.enghttp://creativecommons.org/licenses/by/4.0/Bone implant interactionsSurface chemistryAnimal experimentsBiomaterialsPolyphenolsAlkaline PhosphataseReverse Transcriptase Polymerase Chain ReactionPolyphenolsRabbitsBiocompatible MaterialsTartrate-Resistant Acid PhosphataseL-Lactate DehydrogenaseFemaleAnimalsOsseointegrationRANK Ligandresearch articleAttribution 4.0 International303663831911331910.3390/ijms19113319International Journal of Molecular Sciencesopen accessAnimalesOseointegraciónPrótesis e ImplantesL-Lactato DeshidrogenasaFemeninoReacción en Cadena de la Polimerasa de Transcriptasa InversaConejosLigando RANKMateriales BiocompatiblesPolifenolesFosfatasa AlcalinaQuercetinaRatonesOsteogénesisOsteoclastosFosfatasa ácida Tartratorresistente2-s2.0-85055613213451528500035L624753343