Diaz-Guerra, Teresa M.Mellado, EmiliaCuenca-Estrella, ManuelRodriguez-Tudela, Juan Luis2019-08-132019-08-132003-03Antimicrob Agents Chemother. 2003 Mar;47(3):1120-4.0066-4804http://hdl.handle.net/20.500.12105/8234Erratum in: Antimicrob Agents Chemother. 2004 Mar;48(3):1071.The genes encoding 14alpha-sterol demethylases (cyp51A and cyp51B) were analyzed in 12 itraconazole (ITC)-resistant and three ITC-susceptible clinical isolates of Aspergillus fumigatus. Six ITC-resistant strains exhibited a substitution of another amino acid for glycine at position 54, which is located at a very conserved region of the Cyp51A protein. The cyp51A gene from the A. fumigatus wild-type strain (CM-237) was replaced with the mutated cyp51A gene copy of an ITC-resistant strain (AF-72). Two transformants exhibited resistance to ITC, both of which had incorporated the mutated copy of the cyp51A gene.engAMAmino Acid SubstitutionAntifungal AgentsAspergillus fumigatusBase SequenceCytochrome P-450 Enzyme SystemDrug Resistance, FungalElectroporationFungal ProteinsItraconazoleMicrobial Sensitivity TestsMolecular Sequence DataPoint MutationReverse Transcriptase Polymerase Chain ReactionTransformation, GeneticAtribución-NoComercial-CompartirIgual 4.0 Internacional126045514731120-410.1128/aac.47.3.1120-1124.2003Antimicrobial agents and chemotherapyopen access