Evangelou, KonstantinosLougiakis, NikolaosRizou, Sophia VKotsinas, AthanassiosKletsas, DimitrisMuñoz-Espín, DanielKastrinakis, Nikolaos GPouli, NicoleMarakos, PanagiotisTownsend, PaulSerrano Marugan, ManuelBartek, JiriGorgoulis, Vassilis G2019-03-012019-03-012017-02Aging Cell. 2017;16(1):192-197.14749718http://hdl.handle.net/20.500.12105/7266Cellular senescence contributes to organismal development, aging, and diverse pathologies, yet available assays to detect senescent cells remain unsatisfactory. Here, we designed and synthesized a lipophilic, biotin-linked Sudan Black B (SBB) analogue suitable for sensitive and specific, antibody-enhanced detection of lipofuscin-containing senescent cells in any biological material. This new hybrid histo-/immunochemical method is easy to perform, reliable, and universally applicable to assess senescence in biomedicine, from cancer research to gerontology.engVoRhttp://creativecommons.org/licenses/by/4.0/Sudan Black BAgingBiotin‐linked compoundsImmunohistochemistrySenescenceAnimalsBiological AssayBiomarkersBiotinCell Line, TumorHumansMiceCellular SenescenceAtribución 4.0 Internacional28165661161192-19710.1111/acel.125451474-9726Aging cellopen access