Aguado-Garcia, DianaOlvera, AlexBrander, ChristianSanchez-Merino, VictorYuste, Eloisa2022-11-182022-11-182022-03-22Vaccines (Basel). 2022 Mar 22;10(4):484.2076-393Xhttp://hdl.handle.net/20.500.12105/15192The long-term storage stability of vaccines has a major impact on the roll-out and success of global immunization programs. For the Human Immunodeficiency Virus type 1 (HIV-1) virus-like particle (VLP) vaccine prototype evaluated here, nanoparticle tracking analysis (NTA), and enzyme-linked immunoabsorbent assay (ELISA) results demonstrated a remarkable structural stability. VLPs maintained their integrity and the recognition of relevant B-cell epitopes for three months at 4 and -20 °C. Interestingly, most particles remained intact and preserved the recognition of relevant epitopes even after a week of storage at room temperature.engVoRhttp://creativecommons.org/licenses/by/4.0/Virus-like particlesHIVVaccineThermostabilityAtribución 4.0 Internacional3545523310448410.3390/vaccines10040484Vaccinesopen access