Martin-Cofreces, Noa B.Chichon, F JCalvo, EnriqueTorralba, DanielBustos-Moran, EugenioDosil, Sara GRojas-Gomez, ABonzon-Kulichenko, ElenaLopez, Juan AntonioOton, JSorrentino, AZabala, J CVernos, IsabelleVazquez, JesusValpuesta, JoseSanchez-Madrid, Francisco2021-07-152021-07-152020-12Sci Adv. 2020; 6(49):eabb72422375-2548http://hdl.handle.net/20.500.12105/13242T lymphocyte activation requires the formation of immune synapses (IS) with antigen-presenting cells. The dynamics of membrane receptors, signaling scaffolds, microfilaments, and microtubules at the IS determine the potency of T cell activation and subsequent immune response. Here, we show that the cytosolic chaperonin CCT (chaperonin-containing TCP1) controls the changes in reciprocal orientation of the centrioles and polarization of the tubulin dynamics induced by T cell receptor in T lymphocytes forming an IS. CCT also controls the mitochondrial ultrastructure and the metabolic status of T cells, regulating the de novo synthesis of tubulin as well as posttranslational modifications (poly-glutamylation, acetylation, Δ1 and Δ2) of αβ-tubulin heterodimers, fine-tuning tubulin dynamics. These changes ultimately determine the function and organization of the centrioles, as shown by three-dimensional reconstruction of resting and stimulated primary T cells using cryo-soft x-ray tomography. Through this mechanism, CCT governs T cell activation and polarity.engVoRhttp://creativecommons.org/licenses/by-nc/4.0/Atribución-NoComercial 4.0 Internacional33268369649eabb724210.1126/sciadv.abb7242Science advancesopen access