Otón, JoaquínPereiro, EvaConesa, José JChichón, Francisco JavierLuque, DanielRodriguez Martinez, Javier MPérez-Berná, Ana JSorzano, Carlos Oscar SKlukowska, JoannaHerman, Gabor TVargas, JavierMarabini, RobertoCarrascosa, José LCarazo, José M2019-03-262019-03-262017-04-04Sci Rep. 2017 Apr;7:458082045-2322http://hdl.handle.net/20.500.12105/7365We have developed a new data collection method and processing framework in full field cryo soft X-ray tomography to computationally extend the depth of field (DOF) of a Fresnel zone plate lens. Structural features of 3D-reconstructed eukaryotic cells that are affected by DOF artifacts in standard reconstruction are now recovered. This approach, based on focal series projections, is easily applicable with closed expressions to select specific data acquisition parameters.engVoRhttp://creativecommons.org/licenses/by/4.0/AlgorithmsImage Processing, Computer-AssistedImaging, Three-DimensionalTomography, X-RayAtribución-4.0 Internacional28374769714580810.1038/srep45808Scientific Reportsopen access