Sala-Vila, AleixNavarro-Lerida, InmaculadaSanchez-Alvarez, MiguelBosch, MartaCalvo, CarlosLopez, Juan AntonioCalvo, EnriqueFerguson, CharlesGiacomello, MartaSerafini, AnnalisaScorrano, LucaEnriquez, Jose AntonioBalsinde, JesusParton, Robert G.Vazquez, JesusPol, Albertdel Pozo, Miguel Angel2017-10-302017-10-302016Sci Rep. 2016; 6:273512045-2322http://hdl.handle.net/20.500.12105/5222The mitochondria-associated membrane (MAM) is a specialized subdomain of the endoplasmic reticulum (ER) which acts as an intracellular signaling hub. MAM dysfunction has been related to liver disease. We report a high-throughput mass spectrometry-based proteomics characterization of MAMs from mouse liver, which portrays them as an extremely complex compartment involved in different metabolic processes, including steroid metabolism. Interestingly, we identified caveolin-1 (CAV1) as an integral component of hepatic MAMs, which determine the relative cholesterol content of these ER subdomains. Finally, a detailed comparative proteomics analysis between MAMs from wild type and CAV1-deficient mice suggests that functional CAV1 contributes to the recruitment and regulation of intracellular steroid and lipoprotein metabolism-related processes accrued at MAMs. The potential impact of these novel aspects of CAV1 biology on global cell homeostasis and disease is discussed.engVoRhttp://creativecommons.org/licenses/by/4.0/ER MEMBRANESALZHEIMER-DISEASECELLSCHOLESTEROLDYSFUNCTIONPROTEIN-1DROPLETSAtribución 4.0 Internacional27272971610.1038/srep27351Scientific Reportsopen access