Saez-Nieto, Juan AntonioCarrasco, GemaPino-Rosa, Silvia delMedina-Pascual, Maria JoseGarrido, NoeliaVillalón, PilarValdezate, Sylvia2022-05-092022-05-092021-12New Microbes New Infect. 2021 Dec 13;44:100946.2052-2975http://hdl.handle.net/20.500.12105/14342Two hundred and eighty-six isolates from human clinical samples were identified between 1996 and 2019 as belonging to 8 families, 19 genera and 88 species of Actinobacteria. The most identified genera were Streptomyces (182 strains from 45 species), Actinomadura (29 strains, 5 species), Nocardiopsis (21 strains, 6 species) and Dietzia (18 strains, 5 species). The rest of the identified genera (15) contained 27 species with 36 isolates. Of the species studied, only 13/88 had been documented previously as isolates from clinical samples, and in some cases, as true pathogens. In this sense, a literature review of the species found in infections or in clinical samples without clear involvement in pathology has been carried out. Finally, the susceptibility to 8 antimicrobial agents has been studied. Streptomyces showed high resistance (80.8%) against cefotaxime and cotrimoxazole (55.5%), and no isolate resistance to amikacin and linezolid have been found. Lower percentages of resistance have been found in other genera, except in Dietzia (100% against cotrimoxazole and 44.4% against erythromycin). The greatest resistance in these genera was to cotrimoxazole (29.8) and erythromycin (27,9%), and no resistance to linezolid has been found in these genera. In Microbispora, Nonomuraea and Umezawaea, no resistant isolates have been found against any antibiotic studied. Only 3/104 isolates were resistant to amikacin in Amycolatopsis, Crossiella, and Micromonosopora. One isolate of Amycolatopsis was resistant to imipenem.engVoRhttp://creativecommons.org/licenses/by-nc-nd/4.0/ActimomaduraActinobacteriaStreptomycesAntimicrobial susceptibilityHuman clinical samplesAttribution-NonCommercial-NoDerivatives 4.0 Internacional349173884410094610.1016/j.nmni.2021.100946New Microbes and New Infectionsopen access